PXD041520

PXD041520 is an original dataset announced via ProteomeXchange.

Title	Synthesis of flavonol-bearing probes and chemical proteomic analysis of Asteraceae petals in search of flavonoid enzymes
Description	In flowers of Asteraceae, yellow flavonols bearing an additional hydroxyl group at positions 6 or 8 (ring A) contribute to petal UV-absorbing pigmentation patterns, which play a crucial role in attracting pollinating insects. Understanding the biogenesis of these special flavonols requires the identification of any specific enzyme involved in these incorporations of extra hydroxyl groups on the quercetin ring A. To this aim, flavonol-bearing biotinylated probes have been designed and synthesized to explore their ability to selectively capture target proteins or biosynthetic enzymes under oxidative activation. These probes demonstrate the ability to capture several flavonoid enzymes from Rudbeckia and Tagetes microsomes and allow the identification of uncharacterized candidates for novel flavonoid enzymes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:57.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stéphane CHAIGNEPAIN
SpeciesList	scientific name: Rudbeckia hirta; NCBI TaxID: 52299; scientific name: Tagetes erecta; NCBI TaxID: 13708;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-13 07:57:13	ID requested
1	2023-07-20 12:08:32	announced
2	2023-11-14 09:03:48	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:58	announced	2024-10-22: Updated project metadata.

Kempf K, Kempf O, Capello Y, Molitor C, Lescoat C, Melhem R, Chaignepain S, G, é, not E, Groppi A, Nikolski M, Halbwirth H, Deffieux D, Quideau S, Synthesis of Flavonol-Bearing Probes for Chemoproteomic and Bioinformatic Analyses of Asteraceae Petals in Search of Novel Flavonoid Enzymes. Int J Mol Sci, 24(11):(2023) [pubmed]

10.3390/ijms24119724;

submitter keyword: natural products • plant polyphenols • proteomics • bioinformatics • biosynthesis of higher hydroxylated flavonols

Caroline Tokarski
contact affiliation	Team: Mass spectrometry of biological molecules Institute CBMN for Chemistry and Biology of Membranes and Nano-objects UMR 5248 CNRS-Bordeaux University 33607 Pessac - FRANCE
contact email	c.tokarski@cbmn.u-bordeaux.fr
lab head
Stéphane CHAIGNEPAIN
contact affiliation	CNRS - French National Center for Scientific Research
contact email	s.chaignepain@cbmn.u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041520

PRIDE project URI

• PRIDE
  1. PXD041520
     1. Label: PRIDE project
     2. Name: Synthesis of flavonol-bearing probes and chemical proteomic analysis of Asteraceae petals in search of flavonoid enzymes