PXD041506

PXD041506 is an original dataset announced via ProteomeXchange.

Title	LIPID DROPLET PROTEIN OF SEEDS (LDPS) influences lipid droplet size and number in seeds and seedlings
Description	Lipid droplets (LDs) are subcellular organelles found in all kingdoms of life. While LDs are well known as intracellular depots for neutral lipid storage, there is a growing appreciation that they are much more dynamic in terms of their functions. For instance, recent work in yeast and mammalian systems have revealed that LDs are involved in stress response, protein sequestration, and development, and that these roles are mediated by distinct proteins located on the LD surface. However, few yeast and mammalian LD proteins have obvious homologs in plants. Indeed, relatively few plant LD proteins have been characterized, limiting our overall understanding of the molecular mechanisms underlying LD biogenesis, maintenance, and turnover in plant cells. To address this, we have performed proteomic surveys of LDs isolated from various Arabidopsis tissues, and here we discuss the characterization of one such newly identified LD protein, LIPID DROPLET PROTEIN OF SEEDS (LDPS). LDPS in Arabidopsis is annotated to be of unknown function and is expressed exclusively in developing and mature seeds, as well as in young seedlings. Notably, mature ldps mutant seeds have smaller LDs and less storage oil relative to wild-type seeds, while ectopic overexpression of LDPS in leaves leads to an increase in LD number. Furthermore, following germination, LDs in ldps mutant seedlings do not appear to fuse during their turnover, as they do in wild-type seedlings. Protein-protein interaction assays combined with protein co-expression experiments suggest that LDPS interacts with other known LD proteins, in particular, oleosins. Taken together, these findings and those from proteomics and lipidomics analyses of ldps mutant seeds indicate that LDPS plays a key role in LD biogenesis and regulating LD size and number in plant seeds.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_16:08:37.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-13 01:41:08	ID requested
⏵ 1	2025-06-08 16:08:38	announced

10.1093/plcell/koaf121;

Doner NM, Clews AC, Esnay N, Whitehead PS, Wang Y, Romsdahl TB, Seay D, Niemeyer PW, Bonin M, Xu Y, Valerius O, Braus GH, Ischebeck T, Chapman KD, Dyer JM, Mullen RT, LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings. Plant Cell, 37(5):(2025) [pubmed]

submitter keyword: Arabidopsis, lipid droplets, seeds

Till Ischebeck
contact affiliation	University of Muenster
contact email	till.ischebeck@uni-muenster.de
lab head
Till Ischebeck
contact affiliation	University of Münster
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041506
     1. Label: PRIDE project
     2. Name: LIPID DROPLET PROTEIN OF SEEDS (LDPS) influences lipid droplet size and number in seeds and seedlings