PXD041496

PXD041496 is an original dataset announced via ProteomeXchange.

Title	Nuclear pore complexes mediate subtelomeric gene silencing by regulating PCNA levels on chro atin
Description	The nuclear pore complex (NPC) physically interacts with chromatin and regulates gene expression. The Saccharomyces cerevisiae inner ring nucleoporin Nup170 has been implicated in chromatin organization and the maintenance of gene silencing in subtelomeric regions. To gain insight into how Nup170 regulates this process, we used protein-protein interactions, genetic interactions, and transcriptome correlation analyses to identify the Ctf18-RFC complex, an alternative proliferating cell nuclear antigen (PCNA) loader, as a facilitator of the gene regulatory functions of Nup170. The Ctf18-RFC complex is recruited to a subpopulation of NPCs that lack the nuclear basket proteins Mlp1 and Mlp2. In the absence of Nup170, PCNA levels on DNA are reduced, resulting in the loss of silencing of subtelomeric genes. Increasing PCNA levels on DNA by removing Elg1, which is required for PCNA unloading, rescues subtelomeric silencing defects in nup170Δ. The NPC therefore mediates subtelomeric gene silencing by regulating PCNA levels on DNA.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:47:52.106.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041496
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arti Navare
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-12 16:32:18	ID requested
1	2023-06-12 17:02:57	announced
2	2023-11-14 08:57:55	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:47:52	announced	2024-10-22: Updated project metadata.

10.1083/JCB.202207060;

10.6019/PXD041496;

submitter keyword: Nuclear Pore Complex, Subtelomeric gene silencing, Nup170, Nucleoporin, Ctf18-RFC, PCNA

John D. Aithicson
contact affiliation	Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington 98109, USA Departments of Pediatrics and Biochemistry, University of Washington, Seattle, Washington 98195, USA
contact email	john.aitchison@seattlechildrens.org
lab head
Arti Navare
contact affiliation	Seattle Children's Hospital
contact email	arti.navare@seattlechildrens.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041496

PRIDE project URI

• PRIDE
  1. PXD041496
     1. Label: PRIDE project
     2. Name: Nuclear pore complexes mediate subtelomeric gene silencing by regulating PCNA levels on chro atin