PXD041491

PXD041491 is an original dataset announced via ProteomeXchange.

Title	Growth rate and limiting substrate define the nutritional composition and cell size of microbial biomass for food applications
Description	Microbial biomass has emerged as a promising sustainable protein alternative. The cultivation conditions affect the composition of microbial cells, and hence their quality and nutritional value. Here, we investigated the relationship between growth rate, substrate availability and cell composition and size of Cupriavidus necator and Komagataella phaffii. Biomass with decreased nucleic acid and increased protein content was produced at low growth rates. Conversely, high rates resulted in larger cells, which could enable more efficient biomass harvesting. The proteome allocation varied across the different growth rates, with more ribosomal proteins at higher rates. Considering the distinct amino acid profiles established for the different cellular components, variations in their abundance impacts the product quality leading to higher cysteine and phenylalanine content at low growth rates. In summary, we demonstrate tradeoffs between nutritional quality and production rate, and we discuss that techno-functional food properties should be considered when designing microbial biomass production processes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:22:31.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robbin Bouwmeester
SpeciesList	scientific name: Komagataella phaffii; NCBI TaxID: 460519; scientific name: Cupriavidus necator; NCBI TaxID: 106590;
ModificationList	carbamoylated residue; amidated residue; biotinylated residue; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-12 08:09:18	ID requested
⏵ 1	2024-01-26 10:22:32	announced

Sakarika M, Kerckhof FM, Van Peteghem L, Pereira A, Van Den Bossche T, Bouwmeester R, Gabriels R, Van Haver D, Ul, č, ar B, Martens L, Impens F, Boon N, Ganigu, é R, Rabaey K, The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions. Microb Cell Fact, 22(1):254(2023) [pubmed]

10.1186/s12934-023-02265-1;

submitter keyword: growth rate, microbial protein, substrate availability, proteome allocation,amino acid profile, nucleic acid, nutritional quality, macromolecular cell composition, microbial food, protein profile

Korneel Rabaey
contact affiliation	Center for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering Ghent University, Coupure Links 653, 9000 Ghent, Belgium, Center for Advanced Process Technology for Urban Resource recovery (CAPTURE), Frieda Saeysstraat 1, 9052 Ghent, Belgium
contact email	Korneel.Rabaey@UGent.be
lab head
Robbin Bouwmeester
contact affiliation	VIB-UGent
contact email	robbin.bouwmeester@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD041491

PRIDE project URI

• PRIDE
  1. PXD041491
     1. Label: PRIDE project
     2. Name: Growth rate and limiting substrate define the nutritional composition and cell size of microbial biomass for food applications