PXD041490

PXD041490 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus
Description	Aspergillus fumigatus is the main culprit of invasive aspergillosis, which has a high mortality rate in immunocompromised patients. Lysine 2-hydroxyisobutyrylation is a highly conserved posttranslational modification found in a wide variety of organisms. In this study, we survey the biological impact of 2-hydroxyisobutyrylation on lysine residuals (Khib) in A. fumigatus. Using an antibody-enrichment approach along with the traditional LC-MS/MS method, the pattern of Khib-modified proteins and sites were analyzed in one wild type strain of A. fumigatus. We identified 3494 Khib-modified proteins with 18091 modified sites in this strain, and a more detailed bioinformatics analysis indicated that the Khib-modified proteins are involved in a wide range of cellular functions with diverse subcellular locations. Functional enrichment analysis featured several prominent functional pathways, including ribosome, biosynthesis of amino acids and nucleocytoplasmic transport – of which the ribosome pathway is the most affected pathway. When compared with other reported Khib eukaryotes, both Khib-modified sites and Khib-modified proteins also remained the highest. It proves that this modification is of great importance in A. fumigatus. At the same time, several enzymes in the fungal ergosterol synthesis pathway are modified with Khib. These bioinformatic results suggest that 2-hydroxyisobutyrylation may play an indispensable role in the regulation of the ribosomal biogenesis and the process of fungal resistance. Findings in this study may provide new insights for studying PTM-associated mechanisms in fungal development and antifungal drug development.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:51:05.457.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hailin Zheng
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-12 07:17:41	ID requested
⏵ 1	2024-01-26 07:51:06	announced

10.1007/s00284-023-03565-w;

Zheng H, Mei H, Li X, Li D, Liu W, Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus. Curr Microbiol, 81(3):74(2024) [pubmed]

submitter keyword: Lysine 2-Hydroxyisobutyrylation, Aspergillus fumigatus,posttranslational modification, proteome

HaiLin Zheng
contact affiliation	Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China.
contact email	664661595@qq.com
lab head
Hailin Zheng
contact affiliation	Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu 210042 People’s Republic of China. Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, Jiangsu 210042, People’s Republic of China.
contact email	664661595@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041490
     1. Label: PRIDE project
     2. Name: Proteome-wide analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus