PXD041480
PXD041480 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stress granules protect ER-exit sites from TDP43 aggregation |
| Description | Aggregation of proteins under cellular stress plays key roles in age-related degenerative diseases, but how different proteins coalesce to form inclusions that vary in composition, morphology, molecular dynamics and physiological consequences is poorly understood. We employed a general reporter to identify proteins forming aggregates under proteotoxic stress in human cells. Over 300 proteins were identified, forming different inclusions containing subsets of aggregating proteins. In particular, TDP43, implicated in Amyotrophic Lateral Sclerosis (ALS), partitions dynamically between two distinct types of aggregates: stress granule (SG) and a previously unknown solid inclusion containing components of the ER exit sites (ERES), such as SEC16A. TDP43 accumulation at ERES is antagonized by SG assembly, but enhanced by certain ALS-associated mutations. TDP43-ERES aggregation biases toward nascent TDP43 and, unlike SG, does not contain RNA. Such aggregation causes defects in ER-to-Golgi protein transport, providing a link between TDP43 aggregation and compromised cellular function in ALS patient neurons. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-04-12 |
| AnnouncementXML | Submission_2024-04-11_08:00:16.678.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Radoslaw Sobota |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; unknown modification; unknown modification; deamidated L-asparagine; deamidated L-glutamine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-11 23:17:53 | ID requested | |
| ⏵ 1 | 2024-04-11 08:00:17 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Stress granules, endoplasmic reticulum, TDP43, aggregation |
Contact List
| Rong Li | |
|---|---|
| lab head | |
| Radoslaw Sobota | |
| contact affiliation | IMCB A-STAR Singapore |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002060/ |
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