PXD041470

PXD041470 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteome profiling of a Xanthomonas campestris pv campestris B100 culture grown in a minimal medium with a specific focus on nutrient consumption and xanthan biosynthesis
Description	Xanthan is the most used polysaccharide of bacterial origin. There are many industrial applications for xanthan, especially in food products. The organism Xanthomonas campestris is commonly used for xanthan fermentation, but little is known about the xanthan synthesis mechanism on the proteome level. The study used a label-free LC-MS/MS method to investigate the protein shifts during a typical xanthan fermentation of 72 hours. Two thousand four hundred sixteen proteins were identified, representing 54.75 % of the 4413 entries in the reference database. One thousand seven hundred forty-nine proteins were measured in a quantifiable quality. The quality of the proteome data was examined by the abundance changes of the ribosomal proteins according to the growth phases. The phenotypic changes during the cultivation, especially processes known to be influencing or close related to xanthan production, were investigated by proteome analysis. These processes were nitrogen metabolism, sugar nucleotide metabolism, and xanthan synthesis. This study shows the abundance of changes in central metabolites during a xanthan fermentation of Xcc. The presented research provides crucial information for further biotechnological studies to improve xanthan production.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_02:16:25.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ben Struck
SpeciesList	scientific name: Xanthomonas campestris pv. campestris str. B100; NCBI TaxID: 509169;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-11 07:59:14	ID requested
⏵ 1	2024-05-24 02:16:26	announced

10.3390/proteomes12020012;

Struck B, Wiersma SJ, Ortseifen V, P, ü, hler A, Niehaus K, pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis. Proteomes, 12(2):(2024) [pubmed]

submitter keyword: Quantitative proteomics, Xanthan fermentation, Time course, Xanthomonas campestris pv. campestris B100,Bacterial proteome, LC-MS/MS

Prof. Dr. Karsten Niehaus
contact affiliation	Bielefeld University, Center for Biotechnology CeBiTec,
contact email	kniehaus@cebitec.uni-bielefeld.de
lab head
Ben Struck
contact affiliation	University Bielefeld
contact email	bstruck@cebitec.uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041470
     1. Label: PRIDE project
     2. Name: Comprehensive proteome profiling of a Xanthomonas campestris pv campestris B100 culture grown in a minimal medium with a specific focus on nutrient consumption and xanthan biosynthesis