PXD041450

PXD041450 is an original dataset announced via ProteomeXchange.

Title	Growth factor induced desialylation for the fast control of endocytosis
Description	It is commonly assumed that the glycan makeup of glycoproteins is final and static once these have reached the cell surface. Here, we discovered a molecular switch involving acute changes of glycans at the plasma membrane that challenges this notion. We demonstrate that growth factors tune within minutes the endocytosis of cell surface glycoproteins through a unique mechanism of pH-triggered enzymatic removal of sialic acids. The subsequent retrograde transport of these desialylated glycoproteins to the Golgi apparatus allows for the resetting of their glycan makeup and the repurposing of their functions through polarized re-secretion to specialized plasma membrane areas, such as the leading edge to sustain cell motility. Thus, glycosylation thereby emerges as a dynamic regulatory post-translational modification similar to phosphorylation and ubiquitylation.
HostingRepository	PRIDE
AnnounceDate	2024-11-29
AnnouncementXML	Submission_2024-11-29_01:42:37.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Masson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-11 01:52:43	ID requested
⏵ 1	2024-11-29 01:42:38	announced

Dataset with its publication pending

submitter keyword: Endocytosis, Gal3, sialylation, EGFR

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Vanessa Masson
contact affiliation	Institut Curie - Centre de Recherche
contact email	vanessa.masson@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD041450

PRIDE project URI

• PRIDE
  1. PXD041450
     1. Label: PRIDE project
     2. Name: Growth factor induced desialylation for the fast control of endocytosis