PXD041442

PXD041442 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis between T cell lysate and T cell microvilli-derived particles
Description	Although T cell activation is closely linked to internalization of the T cell antigen receptor (TCR), relatively few studies have examined the release of TCRs via shedding of T cell microvilli following physical interaction with cognate antigen-presenting cells. In this study, we investigated the physiological implications of the release of TCRs and other external membrane components. We term this event “trogocytic molting”, as it occurs through a combined process of trogocytosis and enzymatic vesiculation of microvillar membrane structures. Surprisingly, in contrast to TCR internalization, this event leads to rapid upregulation of surface TCRs and remarkable metabolic reprogramming of cholesterol and fatty acids synthesis to meet the demands of clonal expansion, which drives multiple rounds of division and cell survival. To elucidate a potential mechanism for how trogocytic molting of microvilli enhances T-cell proliferation, purified TISs obtained from activated naive CD3+ T cells were subjected to LC-MS/MS analysis to identify TISs-enriched functional proteins.
HostingRepository	PRIDE
AnnounceDate	2023-05-09
AnnouncementXML	Submission_2023-05-08_20:02:21.445.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WonchangSoh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-10 15:01:05	ID requested
⏵ 1	2023-05-08 20:02:21	announced

Dataset with its publication pending

submitter keyword: extracellular vesicle, proteomics,T cell microvilli

Chang-DukJun
contact affiliation	Immune Synapse and Cell Therapy laboratory, Gwangju institute of science and technology (GIST), South Korea
contact email	cdjun@gist.ac.kr
lab head
WonchangSoh
contact affiliation	GIST (Gwangju institute in science and technology)
contact email	onecsoh94@gm.gist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD041442

PRIDE project URI

• PRIDE
  1. PXD041442
     1. Label: PRIDE project
     2. Name: Proteomics analysis between T cell lysate and T cell microvilli-derived particles