PXD041415

PXD041415 is an original dataset announced via ProteomeXchange.

Title	Proteomic shift in mouse embryonic fibroblasts Pfa1 during L-Buthionine-sulfoximine (BSO) induced ferroptosis
Description	This report provides data on ferroptosis induced proteomic response in immortalized mouse embryonic fibroblasts which are 4-OH-TAM-inducible Gpx4−/− (referred to as Pfa1 cells). Pfa1 cells are a valuable, widespread and well characterized model for ferroptosis research. We used L-Buthionine-sulfoximine (BSO) to induce ferroptosis and collected samples at 0h, 24h and 48 h after treatment. Untreated cells were used as a control. Pfa1 cells (kind gift from Marcus Conrad, Munich) were cultured in RPMI-1640 media (2 g/L glucose, 10% FBS, 2 mM Gibco GlutaMAX Supplement, 1% pen/strep) at 37C with 5% CO2 in a humidified incubator. Cells (300k) were seeded on Corning 100 mm tissue-culture treated culture dishes and incubated overnight. On the next day Pfa1 cells were treated with or without 30 µM BSO.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:41:26.891.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041415
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Olga Kudryashova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-09 02:43:57	ID requested
1	2023-04-12 05:59:54	announced
2	2023-07-24 02:38:49	announced	2023-07-24: Updated project metadata.
⏵ 3	2023-11-14 06:41:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD041415;

submitter keyword: BSO, proteomics, mouse embryonic fibroblasts,Ferroptosis, erastin, glutathione peroxidase 4, L-Buthionine-sulfoximine, Pfa1, cell death

Dr Dr Vsevolod V. Belousov
contact affiliation	Federal Center of Brain Research and Neurotechnologies, Federal Medico-Biological Agency, 117513 Moscow, Russia
contact email	vsevolod.belousov@gmail.com
lab head
Olga Kudryashova
contact affiliation	Federal Center of Brain Research and Neurotechnologies
contact email	olga.kudryashova179@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD041415

PRIDE project URI

• PRIDE
  1. PXD041415
     1. Label: PRIDE project
     2. Name: Proteomic shift in mouse embryonic fibroblasts Pfa1 during L-Buthionine-sulfoximine (BSO) induced ferroptosis