PXD041406

PXD041406 is an original dataset announced via ProteomeXchange.

Title	Ralstonia solanacearum core effector RipE1 interacts and cleaves the Arabidopsis exocyst component Exo70B1
Description	Ralstonia solanacearum depends on numerous virulence factors, also known as effectors, to promote disease in a wide range of economically important host-plants. Although some of these effectors have been characterized, none has been shown to target the host’s secretion machinery so far. Here, a screening was performed, using an extended library of NLR plant immune receptors’ integrated domains (IDs), to identify new effector targets. The results uncovered that the core effector RipE1, of the R. solanacearum species complex, among other targets, associates with Arabidopsis exocyst component Exo70B1. RipE1, in accordance with its predicted cysteine protease activity, cleaves Exo70B1 in vitro between its sequence and is able to promote Exo70B1 degradation in planta. RipE1 enzymatic activity additionally results in the activation of TN2-dependent cell death. TN2 is an atypical NLR that has been proposed to guard Exo70B1. Despite the fact that RipE1 has been previously reported to activate defense responses in model plant species, we present here a Nicotiana species, in which RipE1 expression does not activate cell death. Overall, this study uncovers a new RipE1 host target, while providing evidence and novel tools to advance in-depth studies of RipE1 and homologues effectors.
HostingRepository	PRIDE
AnnounceDate	2025-04-07
AnnouncementXML	Submission_2025-04-07_06:29:15.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikos Kountourakis
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-07 08:59:00	ID requested
⏵ 1	2025-04-07 06:29:16	announced

10.1016/J.ISCI.2025.112307;

submitter keyword: Pathogen effector, T3SS, Cysteine protease, exocyst complex, T3E, Plant defense

Panagiotis F. Sarris
contact affiliation	1 Department of Biology, University of Crete, 714 09 Heraklion, Crete, Greece 2 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece
contact email	p.sarris@uoc.gr
lab head
Nikos Kountourakis
contact affiliation	Institute of Molecular Biology and Biotechnology
contact email	kountour@imbb.forth.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD041406

PRIDE project URI

• PRIDE
  1. PXD041406
     1. Label: PRIDE project
     2. Name: Ralstonia solanacearum core effector RipE1 interacts and cleaves the Arabidopsis exocyst component Exo70B1