PXD041405

PXD041405 is an original dataset announced via ProteomeXchange.

Title	The functional and structural characterization of Xanthomonas campestris pv. campestris core effector XopP revealed a new kinase activity
Description	The exocyst complex subunit protein Exo70B1 plays a crucial role in a variety of cell mechanisms including immune responses against pathogens. The calcium dependent kinase 5 (CPK5) of Arabidopsis thaliana, phosphorylates AtExo70B1 upon functional disruption. We previously reported that, the Xanthomonas campestris pv. campestris effector XopP, compromises AtExo70B1 and bypasses the host’s hypersensitive response (HR), in a way that is still unclear. Herein we designed an experimental approach based on biophysical, biochemical and molecular assays, based on structural and functional predictions, as well as, utilizing Aplhafold and DALI online servers respectively, in order to characterize the in vivo XccXopP function. The interaction between AtExo70B1 and XccXopP is very stable in high temperatures, while the AtExo70B1 appeared to be phosphorylated at XccXopP expressing transgenic Arabidopsis. XccXopP reveals similarities with known mammalian kinases, and phosphorylates AtExo70B1 at Ser107, Ser111, Ser248, Thr309 and Thr364. Moreover, XccXopP protects AtExo70B1 from AtCPK5 phosphorylation. Together these findings show that, XccXopP is an effector, which not only functions as a novel serine/threonine kinase upon its host’s protein target AtExo70B1, but also protects the latter from the innate AtCPK5 phosphorylation, to bypass the host’s immune responses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:46.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikos Kountourakis
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-07 08:39:38	ID requested
1	2023-06-26 05:32:53	announced
⏵ 2	2023-11-14 09:01:55	announced	2023-11-14: Updated project metadata.

Kotsaridis K, Michalopoulou VA, Tsakiri D, Kotsifaki D, Kefala A, Kountourakis N, Celie PHN, Kokkinidis M, Sarris PF, The functional and structural characterization of Xanthomonas campestris pv. campestris core effector XopP revealed a new kinase activity. Plant J, 116(1):100-111(2023) [pubmed]

submitter keyword: T3SS, novel kinase function, exocyst complex,Protein structure prediction, plant defense, Exo70B1, pathogen XopP effector

Panagiotis F. Sarris
contact affiliation	1 Department of Biology, University of Crete, 714 09 Heraklion, Crete, Greece 2 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece
contact email	p.sarris@uoc.gr
lab head
Nikos Kountourakis
contact affiliation	Institute of Molecular Biology and Biotechnology
contact email	kountour@imbb.forth.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041405
     1. Label: PRIDE project
     2. Name: The functional and structural characterization of Xanthomonas campestris pv. campestris core effector XopP revealed a new kinase activity