PXD041401

PXD041401 is an original dataset announced via ProteomeXchange.

Title	A biotin targeting chimera (BioTAC) system to map small molecule interactomes in situ
Description	Understanding how small molecules bind to specific protein complexes in living cells is critical to understanding their mechanism-of-action. Unbiased chemical biology strategies for direct readout of protein interactome remodelling by small molecules would provide advantages over target-focused approaches, including the ability to detect previously unknown ligand targets and complexes. However, there are few current methods for unbiased profiling of small molecule interactomes. To address this, we envisioned a technology that would combine the sensitivity and live-cell compatibility of proximity labelling coupled to mass spectrometry, with the specificity and unbiased nature of photoaffinity labelling. In this manuscript, we describe the BioTAC system, a small-molecule guided proximity labelling platform that can rapidly identify both direct and complexed small molecule binding proteins. We benchmark the system against µMap, photoaffinity labelling, affinity purification coupled to mass spectrometry and proximity labelling coupled to mass spectrometry datasets. We also apply the BioTAC system to provide interactome maps of Trametinib and analogues. The BioTAC system overcomes a limitation of current approaches and supports identification of both inhibitor bound and molecular glue bound complexes.
HostingRepository	PRIDE
AnnounceDate	2023-12-07
AnnouncementXML	Submission_2023-12-06_17:11:39.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Tao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; carboxylated residue; ubiquitinylated lysine; dehydrated residue; biotinylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-07 02:45:40	ID requested
⏵ 1	2023-12-06 17:11:40	announced

10.1038/S41467-023-43507-5;

submitter keyword: LC-MSMS

Fleur Ferguson
contact affiliation	University of California, San Diego
contact email	fmferguson@ucsd.edu
lab head
Andrew Tao
contact affiliation	University of California, San Diego
contact email	ajtao@ucsd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041401
     1. Label: PRIDE project
     2. Name: A biotin targeting chimera (BioTAC) system to map small molecule interactomes in situ