PXD041378

PXD041378 is an original dataset announced via ProteomeXchange.

Title	APEX proximity labeling of murine NDUFS4 interacting proteins in podocytes
Description	In this project we used an unbiased approach to identify the proteins that interact with NDUFS4 in the mouse podocytes using the APEX2 proximity labeling system. An immortalized murine podocyte was permanently transduced with a doxycycline (DOX) inducible NDUFS4-APEX2 chimeric construct. The cells were treated with DOX for 72 h to induce NDUFS4-APEX2 expression, followed by H2O2 activation of APEX2 mediated biotinylation of neighboring proteins. Biotinylated proteins were pull down by streptavidin beads and analyzed by LC-MS/MS. Cells without DOX induction or with DOX but without H2O2 activation were used as controls
HostingRepository	PRIDE
AnnounceDate	2024-02-08
AnnouncementXML	Submission_2024-02-08_09:49:20.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antrix Jain
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-06 06:48:37	ID requested
⏵ 1	2024-02-08 09:49:20	announced

Dataset with its publication pending

submitter keyword: APEX proximity labeling of murine NDUFS4 interacting proteins in podocytes

Farhad R. Danesh
contact affiliation	Division of Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX
contact email	fdanesh@mdanderson.org
lab head
Antrix Jain
contact affiliation	Baylor College of Medicine
contact email	antrixj@bcm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041378
     1. Label: PRIDE project
     2. Name: APEX proximity labeling of murine NDUFS4 interacting proteins in podocytes