⮝ Full datasets listing

PXD041369

PXD041369 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA chemoproteomics approach to discover regulators of innate immune signaling
DescriptionViral dsRNA binds to Retinoic acid Inducible Gene I (RIG-I) Like Receptors (RLRs), promoting the production of Interferon (IFN). Interferon then stimulates the innate and adaptive immune system in an autocrine and paracrine manner. Outside of conical pathways, regulators of the interferon (IFN) activation/response system are poorly characterized. In this study, we used a discovery-biased approach to identify Kinases that are part of the interferon system. Differential changes in phosphorylation sites, in the context of dsRNA RIG-I stimulation, were identified with unbiased mass-spec biased phospho-proteomics. We then computationally identified several Kinases upregulated after RIG-I stimulation from phospho-proteomics data. A Chemoproteomics screen was then used to characterize the altered interferon response in the presence of Kinases inhibitors for the upregulated kinases. Combining unbiased phosphoproteomics with a chemoproteomics screen, we identified several potentially novel regulators of the Interferon system whose inhibition blocked the production of Interferon Stimulated Genes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:46:30.369.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeffrey Johnson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-05 23:25:30ID requested
12023-06-02 14:41:39announced
22023-11-14 08:48:40announced2023-11-14: Updated project metadata.
32024-10-22 05:46:31announced2024-10-22: Updated project metadata.
Publication List
10.3390/v15051112;
Kurland AP, Bonaventure B, Johnson JR, A Chemical Proteomics Approach to Discover Regulators of Innate Immune Signaling. Viruses, 15(5):(2023) [pubmed]
Keyword List
submitter keyword: ATR, ATM, interferon, AMPK, signaling pathways,proteomics
phosphoproteomics, kinases, PLK1, RIG-I
Contact List
Jeffrey R. Johnson
contact affiliationMicrobiology department at Icahn School of Medicine at Mount Sinai
contact emailjeffrey.johnson@mssm.edu
lab head
Jeffrey Johnson
contact affiliationIcahn School of Medicine at Mount Sinai
contact emailjeffrey.johnson@mssm.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041369
PRIDE project URI
Repository Record List
[ + ]