PXD041352

PXD041352 is an original dataset announced via ProteomeXchange.

Title	LC-MS of interacting partners Toxoplasma gondii GRA57
Description	We identified GRA57 as a Toxoplasma gondii secreted effector required for parasite survival of interferon gamma in HFFs. To identify interaction partners of GRA57 during infection of HFFs, which could indicate how GRA57 functions to protect the parasite, we performed co-immunoprecipitation (IP) experiments with an endogenously tagged RHGRA57-HA strain.To enable detection of protein-protein interactions specific to activated cells, HFFs were pre-stimulated before infection with 2.5 U/ml IFN for 6 h, which we found was sufficient to induce IFN responses while retaining host cell viability. HFFs were infected for 24 h with either RH∆Ku80 or RHGRA57-HA parasites prior to lysis. GRA57-HA was immunoprecipitated from lysates, then co-immunoprecipitated proteins were identified by liquid chromatography (LC)-tandem mass spectrometry (MS/MS).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:45:25.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Howell
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-05 07:44:03	ID requested
1	2023-06-21 02:17:50	announced
⏵ 2	2023-11-14 06:45:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, HFF, effectors, dense granule

Moritz Treeck
contact affiliation	The Francis Crick Institute, London, UK & Cell Biology of Host-Pathogen Interaction Laboratory, Instituto Gulbenkian Ciência, Oeiras, Portugal
contact email	moritz.treeck@crick.ac.uk
lab head
Steven Howell
contact affiliation	Francis Crick Institute
contact email	steven.howell@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041352

PRIDE project URI

• PRIDE
  1. PXD041352
     1. Label: PRIDE project
     2. Name: LC-MS of interacting partners Toxoplasma gondii GRA57