PXD041348

PXD041348 is an original dataset announced via ProteomeXchange.

Title	Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
Description	S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a comprehensive multi-omics study was performed on industrially relevant CHO-K1 GS cells throughout a fed-batch process, including metabolomic and proteomic profiling combined with multivariate data and pathway analyses. Multi-layered data and enzymatical assays revealed an intracellular SSC/glutathione mixed disulfide formation and glutaredoxin-mediated reduction, releasing Cys and sulfur species. Increased Cys availability was directed towards glutathione and taurine synthesis, while other Cys catabolic pathways were likewise affected, indicating the cells strive to maintain Cys homeostasis and cellular functions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:00:27.458.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041348
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Melanie Nguyen
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-05 04:49:47	ID requested
1	2023-08-03 12:03:21	announced
⏵ 2	2023-11-14 07:00:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD041348;

submitter keyword: redox control, omics, CHO,S-sulfocysteine, biological activity

Dr. Aline Zimmer
contact affiliation	Upstream R&D, Merck Life Science KGaA, Frankfurter Straße 250, 64293 Darmstadt, Germany
contact email	aline.zimmer@merckgroup.com
lab head
Melanie Nguyen
contact affiliation	Merck Life Science KGaA
contact email	melanie.nguyen@external.merckgroup.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041348
     1. Label: PRIDE project
     2. Name: Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach