PXD041341
PXD041341 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Resiliance and proteome response of Escherichia coli to high levels of isoleucine mistranslation |
Description | Correct pairing of amino acids and tRNA is prerequisite for correct translation of genetic information during protein biosynthesis. Here, we present effects of proteome-wide isoleucine mistranslation in Escherichia coli induced by editing-defective isoleucyl-tRNA synthetase (IleRS). Two types of mistranslation were investigated: substitution of isoleucine (Ile) with either canonical (valine) or noncanonical (norvaline) amino acid. The effects of Ile to Val and Ile to Nva mistranslation were explored on proteome level. High level of Ile substitutions were achieved in editing-deficient strain, leading to excessive protein aggregation. Protein aggregates were analized by LC-MS/MS. Also, interaction of mistranslated proteins with DnaK was explored. The DnaK clients were isolated by pull-down, using endogenously expressed His-tagged DnaK. |
HostingRepository | jPOST |
AnnounceDate | 2024-02-26 |
AnnouncementXML | Submission_2024-02-26_04:16:15.493.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marko Mocibob |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | Q Exactive HF; Q Exactive Plus; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-04-04 22:59:32 | ID requested | |
⏵ 1 | 2024-02-26 04:16:16 | announced |
Publication List
Pranjic M, Sp, รค, t P, Semanjski Curkovic M, Macek B, Gruic-Sovulj I, Mocibob M, Resilience and proteome response of Escherichia coli to high levels of isoleucine mistranslation. Int J Biol Macromol, 262(Pt 1):130068(2024) [pubmed] |
Keyword List
submitter keyword: mistranslation, Escherichia coli, isoleucine, norvaline, aggregation, DnaK, chaperone, translation, aminoacyl-trna synthetase |
Contact List
Ita Gruic Sovulj | |
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lab head | |
Marko Mocibob | |
contact affiliation | University of Zagreb, Faculty of Science |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002117/ |