PXD041341

PXD041341 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Resiliance and proteome response of Escherichia coli to high levels of isoleucine mistranslation
Description	Correct pairing of amino acids and tRNA is prerequisite for correct translation of genetic information during protein biosynthesis. Here, we present effects of proteome-wide isoleucine mistranslation in Escherichia coli induced by editing-defective isoleucyl-tRNA synthetase (IleRS). Two types of mistranslation were investigated: substitution of isoleucine (Ile) with either canonical (valine) or noncanonical (norvaline) amino acid. The effects of Ile to Val and Ile to Nva mistranslation were explored on proteome level. High level of Ile substitutions were achieved in editing-deficient strain, leading to excessive protein aggregation. Protein aggregates were analized by LC-MS/MS. Also, interaction of mistranslated proteins with DnaK was explored. The DnaK clients were isolated by pull-down, using endogenously expressed His-tagged DnaK.
HostingRepository	jPOST
AnnounceDate	2024-02-26
AnnouncementXML	Submission_2024-02-26_04:16:15.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Mocibob
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive HF; Q Exactive Plus; Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-04 22:59:32	ID requested
⏵ 1	2024-02-26 04:16:16	announced

Publication List

Pranjic M, Sp, ä, t P, Semanjski Curkovic M, Macek B, Gruic-Sovulj I, Mocibob M, Resilience and proteome response of Escherichia coli to high levels of isoleucine mistranslation. Int J Biol Macromol, 262(Pt 1):130068(2024) [pubmed]

Pranjic M, Sp, ä, t P, Semanjski Curkovic M, Macek B, Gruic-Sovulj I, Mocibob M, Resilience and proteome response of Escherichia coli to high levels of isoleucine mistranslation. Int J Biol Macromol, 262(Pt 1):130068(2024) [pubmed]

Keyword List

submitter keyword: mistranslation, Escherichia coli, isoleucine, norvaline, aggregation, DnaK, chaperone, translation, aminoacyl-trna synthetase

submitter keyword: mistranslation, Escherichia coli, isoleucine, norvaline, aggregation, DnaK, chaperone, translation, aminoacyl-trna synthetase

Contact List

Ita Gruic Sovulj
lab head
Marko Mocibob
contact affiliation	University of Zagreb, Faculty of Science
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002117/