PXD041327 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic shift in mouse embryonic fibroblasts Pfa1 during ML210-induced ferroptosis |
Description | This report provides data on ferroptosis induced proteomic response in immortalized mouse embryonic fibroblasts which are 4-OH-TAM-inducible Gpx4−/− (referred to as Pfa1 cells). Pfa1 cells are a valuable, widespread and well characterized model for ferroptosis research. We used ML210 (SML0521, Sigma-Aldrich) to induce ferroptosis and collected samples at 0h, 24h and 48 h after treatment. Untreated cells were used as a control. Pfa1 cells (kind gift from Marcus Conrad, Munich) were cultured in RPMI-1640 media (2 g/L glucose, 10% FBS, 2 mM Gibco GlutaMAX Supplement, 1% pen/strep) at 37C with 5% CO2 in a humidified incubator. Cells (300k) were seeded on Corning 100 mm tissue-culture treated culture dishes and incubated overnight. On the next day Pfa1 cells were treated with or without 0.3 µM ML210. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:41:27.140.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041327 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Olga Kudryashova |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-04 06:20:13 | ID requested | |
1 | 2023-07-10 07:20:59 | announced | |
2 | 2023-07-24 02:38:36 | announced | 2023-07-24: Updated project metadata. |
⏵ 3 | 2023-11-14 06:41:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: proteomics, mouse embryonic fibroblasts,Ferroptosis, ML210, erastin, glutathione peroxidase 4, Pfa1, cell death |
Contact List
Dr Vsevolod V. Belousov |
contact affiliation | Federal Center of Brain Research and Neurotechnologies, Federal Medico-Biological Agency, 117513 Moscow, Russia |
contact email | vsevolod.belousov@gmail.com |
lab head | |
Olga Kudryashova |
contact affiliation | Federal Center of Brain Research and Neurotechnologies |
contact email | olga.kudryashova179@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041327
- Label: PRIDE project
- Name: Proteomic shift in mouse embryonic fibroblasts Pfa1 during ML210-induced ferroptosis