PXD041325

PXD041325 is an original dataset announced via ProteomeXchange.

Title	Metabolic enhancement of developmental pausing (embryos)
Description	Embryonic stem (ES) cells and embryos reversibly pause via chemical mTOR inhibition. In this study, we investigate the tissue-specific response to mTORi-induced pausing in ES and trophoblast stem (TS) cells. To resolve the sequential rewiring of the proteome, we conducted a time-series proteomics experiment at 1, 3, 6, 12, 24, and 48 hours upon induction of pausing, and at 1, 3, 6, 12, 24, and 48 hours upon release of pausing in ES and TS cells. We find that ES, but not TS cells pause reversibly. To optimise developmental pausing conditions, we reasoned that by understanding the difference in pausing response of ES and TS cells, we could identify which pathways are essential for pausing. We found that KEGG pathways related to amino acid degradation, fatty acid degradation, and DNA repair are upregulated in ES cells, but downregulated in TS cells during entry into pausing. Moreover, by targeted metabolomics, we found a depletion of short chain carnitines in the paused ES cells. To extend the length of developmental pausing, we supplemented paused embryos with L-carnitine. The L-carnitine supplementation facilitates lipid usage and prolongs the pausing length by 19 days through the establishment of a more dormant state.
HostingRepository	PRIDE
AnnounceDate	2023-11-06
AnnouncementXML	Submission_2023-11-06_02:26:07.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DavidMeierhofer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-04 04:39:36	ID requested
⏵ 1	2023-11-06 02:26:08	announced

Dataset with its publication pending

submitter keyword: pluripotency, mTOR inhibition, embryonic stem cells,embryonic diapause

AydanBulut-Karslioglu
contact affiliation	Max-Planck-Institut für molekulare Genetik Aydan Bulut-Karslioglu, PhD Ihnestraße 63-73 14195 Berlin, Germany
contact email	aydan.karslioglu@molgen.mpg.de
lab head
DavidMeierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD041325

PRIDE project URI

• PRIDE
  1. PXD041325
     1. Label: PRIDE project
     2. Name: Metabolic enhancement of developmental pausing (embryos)