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PXD041324

PXD041324 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNew ruthenium-cyclopentadienyl complexes affect colorectal cancer hallmarks showing high therapeutic potential
DescriptionColorectal cancer (CRC) is one of the most lethal cancers worldwide. Current therapeutic strategies are accompanied by low success rates and several side effects, representing a relevant clinical problem and requiring the discovery of new and more effective therapeutic alternatives. Ruthenium drugs emerged as one of the most promising metallodrugs due to their high selectivity to cancer cells. In this work, we studied for the first time the anticancer properties and mechanisms of action of four lead Ru-cyclopentadienyl compounds, namely PMC79, PMC78, LCR134 and LCR220, in CRC-derived cell lines. Biological assays were performed in CRC cell lines to evaluate cellular distribution, colony formation, cell cycle, proliferation, apoptosis, motility as well as cytoskeleton and mitochondrial alterations. Our results showed that all compounds displayed high bioactivity and selectivity, as shown by low IC50 concentrations against CRC cells. We observed that all Ru compounds had different intracellular distributions and inhibited to a high extent the clonogenic ability and proliferation of CRC cells. In addition, PMC79, LCR134 and LCR220 induced apoptosis, increased the levels of reactive oxygen species, induced mitochondrial alterations and affected F-actin cytoskeleton organization and cellular motility. A proteomic analysis showed that these compounds induce alterations in several cellular proteins related to the phenotypic alterations induced. Overall, we demonstrated that Ru compounds, in special PMC79 and LCR220, present promising anticancer activity in CRC cells and potential to be used as new metallodrugs for CRC therapy.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:03:53.400.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEduard Sabidó
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-04 04:37:52ID requested
12023-07-20 12:32:02announced
22023-11-14 09:03:55announced2023-11-14: Updated project metadata.
Publication List
Br, á, s AR, Fernandes P, Moreira T, Morales-Sanfrutos J, Sabid, ó E, Antunes AMM, Valente A, Preto A, New Ruthenium-Cyclopentadienyl Complexes Affect Colorectal Cancer Hallmarks Showing High Therapeutic Potential. Pharmaceutics, 15(6):(2023) [pubmed]
Keyword List
submitter keyword: ruthenium-cyclopentadienyl compounds
colorectal cancer
active targeting
passive targeting
Contact List
Eduard Sabidó
contact affiliationCenter for Genomic Regulation, Universitat Pompeu Fabra
contact emaileduard.sabido@crg.eu
lab head
Eduard Sabidó
contact affiliationCentre de Regulació Genòmica
contact emaileduard.sabido@crg.cat
dataset submitter
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