PXD041321

PXD041321 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of mouse brain pyruvate kinase binding proteins: a hint for moonlighting functions of PKM1?
Description	Affinity based proteomic profiling is widely used for identification of proteins involved in for-mation of various interactomes. Pyruvate kinase (PK), a classical glycolytic enzyme catalyzing the last step of glycolysis, exists in four isoforms, one of which, PKM2; pyruvate kinase muscle isoform M2 (expressed in actively dividing cells), exhibits many non-canonical (moonlighting) functions. Although the other muscle isoform, PKM1, predominantly expressed in adult differ-entiated tissues, lacks the moonlighting functions, certain evidence exists that it can also perform some non-canonical functions. In order to evaluate the proteomic profile of mouse brain pro-teins bound to PKM1, we have combined in this study affinity-based separation of mouse brain proteins followed by their mass spectrometry identification. Using the highly purified rabbit muscle pyruvate kinase, PKM1 and a 32-mer peptide (PK peptide), sharing high sequence ho-mology with the interface contact region of all PK isoforms, common proteins bound to both af-finity ligands have been recognized. Quantitative affinity binding to the affinity ligands of se-lected identified proteins was validated using a surface plasmon resonance (SPR) biosensor. Bi-oinformatic analysis has shown that the identified proteins, bound to both full length PKM1 and the PK-peptide, form a protein network (interactome). Some of these interactions are relevant for noncanonical (moonlighting) functions of PKM1
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:46.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Tikhonova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-04 03:29:38	ID requested
1	2023-05-10 14:50:00	announced
⏵ 2	2023-11-14 08:48:09	announced	2023-11-14: Updated project metadata.

Buneeva O, Kopylov A, Gnedenko O, Medvedeva M, Veselovsky A, Ivanov A, Zgoda V, Medvedev A, Proteomic Profiling of Mouse Brain Pyruvate Kinase Binding Proteins: A Hint for Moonlighting Functions of PKM1? Int J Mol Sci, 24(8):(2023) [pubmed]

submitter keyword: pyruvapyruvate kinase

pyruvate kinase isoforms

PKM

non-canonical moonlighting functions

PKM-binding proteins

interactomete kinase

pyruvate kinase isoforms

PKM

non-canonical moonlighting functions

PKM-binding proteins

interactome

Olga Tikhonova
contact affiliation	Head of "Human proteome" Core Facility Center, Institute of Biomedical Chemistry
contact email	ovt.facility@gmail.com
lab head
Olga Tikhonova
contact affiliation	Institute of Biomedical Chemistry
contact email	ovt.facility@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041321
     1. Label: PRIDE project
     2. Name: Proteomic profiling of mouse brain pyruvate kinase binding proteins: a hint for moonlighting functions of PKM1?