PXD041310 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation |
Description | The gasotransmitter hydrogen sulfide (H2S) is thought to be involved in the post-translational modification of cysteine residues to produce reactive persulfides. A persulfide-specific chemoselective proteomics approach with mammalian cells has identified a broad range of zinc finger (ZF) proteins as targets of persulfidation. Parallel studies with isolated ZFs show that persulfidation is mediated by Zn(II), O2, and H2S, with intermediates involving oxygen- and sulfur-based radicals detected by mass spectrometry and optical spectroscopies. A small molecule Zn(II) complex exhibits analogous reactivity with H2S and O2, giving a persulfidated product. These data show that Zn(II) is not just a biological structural element, but also plays a critical role in mediating H2S-dependent persulfidation. ZF persulfidation appears to be a general post-translational modification and a possible conduit for H2S signaling. This work has implications for our understanding of H2S-mediated signaling and the regulation of ZFs in cellular physiology and development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:47:12.563.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041310 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Thibaut Vignane |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-03 10:47:19 | ID requested | |
1 | 2024-06-23 08:22:56 | announced | |
⏵ 2 | 2024-10-22 06:47:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Zinc-sulfide,Zinc-finger, H2S, Persulfidation |
Contact List
Milos Filipovic |
contact affiliation | Sulfaging group Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Bunsen-Kirchhoff-Straße 11, 44139 Dortmund Germany |
contact email | thibaut.vignane@isas.de |
lab head | |
Thibaut Vignane |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften - ISAS - eV |
contact email | thibaut.vignane@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041310
- Label: PRIDE project
- Name: Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation