PXD041310

PXD041310 is an original dataset announced via ProteomeXchange.

Title	Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation
Description	The gasotransmitter hydrogen sulfide (H2S) is thought to be involved in the post-translational modification of cysteine residues to produce reactive persulfides. A persulfide-specific chemoselective proteomics approach with mammalian cells has identified a broad range of zinc finger (ZF) proteins as targets of persulfidation. Parallel studies with isolated ZFs show that persulfidation is mediated by Zn(II), O2, and H2S, with intermediates involving oxygen- and sulfur-based radicals detected by mass spectrometry and optical spectroscopies. A small molecule Zn(II) complex exhibits analogous reactivity with H2S and O2, giving a persulfidated product. These data show that Zn(II) is not just a biological structural element, but also plays a critical role in mediating H2S-dependent persulfidation. ZF persulfidation appears to be a general post-translational modification and a possible conduit for H2S signaling. This work has implications for our understanding of H2S-mediated signaling and the regulation of ZFs in cellular physiology and development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:12.563.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041310
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-03 10:47:19	ID requested
1	2024-06-23 08:22:56	announced
⏵ 2	2024-10-22 06:47:12	announced	2024-10-22: Updated project metadata.

10.1002/ANIE.202401003;

10.6019/PXD041310;

submitter keyword: Zinc-sulfide,Zinc-finger, H2S, Persulfidation

Milos Filipovic
contact affiliation	Sulfaging group Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Bunsen-Kirchhoff-Straße 11, 44139 Dortmund Germany
contact email	thibaut.vignane@isas.de
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - eV
contact email	thibaut.vignane@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041310
     1. Label: PRIDE project
     2. Name: Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation