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PXD041298

PXD041298 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry-based proteomic analysis of virions and fibrils from Mimiviridae
DescriptionMimivirus 1.2Mb genome is organized into a 30 nm nucleocapsid-like structure made of two closely related GMC-oxidoreductases, also composing the fibrils decorating its virions. In this work, we used MS-proteomics to characterize the protein content of virions and fibrils from different members of the Mimiviridae family (clade A: Mimivirus reunion -Mr- and Mimivirus M4 -M4, clade B: Moumouvirus australiensis -Ma- and Moumouvirus maliensis -Mm, clade C: Megavirus chilensis -Mc- and Megavirus vitis -Mv). Furthermore, we analyzed fractions purified from Mr mutants devoid of one of the two GMC-oxidoreductases (Mr_KOqu_143 and Mr_KOqu_946), or of both GMC-oxidoreductases (Mr_2KO) with or without expression of the GFP fused to the N-terminus of one GMC-oxidoreductase (Mr_2KO-GFP). Our results show the versatility of the protein content of the fibrils, with fibrils composed of different proteins inter- and even intra-clade, clades B and C viruses presenting fibrils with a protein composition closer to each other than that of clade A viruses.
HostingRepositoryPRIDE
AnnounceDate2024-06-16
AnnouncementXMLSubmission_2024-06-16_01:28:53.112.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD041298
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYohann Couté
SpeciesList scientific name: Mimivirus; NCBI TaxID: 315393; scientific name: Acanthamoeba castellanii; NCBI TaxID: 5755;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-03 01:44:09ID requested
12024-06-16 01:28:53announced
Publication List
Alempic JM, Bisio H, Villalta A, Santini S, Lartigue A, Schmitt A, Bugnot C, Notaro A, Belmudes L, Adrait A, Poirot O, Ptchelkine D, De Castro C, Cout, é Y, Abergel C, Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes. Microlife, 5():uqae006(2024) [pubmed]
10.1093/femsml/uqae006;
10.6019/PXD041298;
Keyword List
submitter keyword: Mimivirus, fibrils, virion, nanoLC-MS/MS
Contact List
Yohann Couté
contact affiliationEDyP
contact emailyohann.coute@cea.fr
lab head
Yohann Couté
contact affiliationEDyP
contact emailyohann.coute@cea.fr
dataset submitter
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Dataset FTP location
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