PXD041298

PXD041298 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based proteomic analysis of virions and fibrils from Mimiviridae
Description	Mimivirus 1.2Mb genome is organized into a 30 nm nucleocapsid-like structure made of two closely related GMC-oxidoreductases, also composing the fibrils decorating its virions. In this work, we used MS-proteomics to characterize the protein content of virions and fibrils from different members of the Mimiviridae family (clade A: Mimivirus reunion -Mr- and Mimivirus M4 -M4, clade B: Moumouvirus australiensis -Ma- and Moumouvirus maliensis -Mm, clade C: Megavirus chilensis -Mc- and Megavirus vitis -Mv). Furthermore, we analyzed fractions purified from Mr mutants devoid of one of the two GMC-oxidoreductases (Mr_KOqu_143 and Mr_KOqu_946), or of both GMC-oxidoreductases (Mr_2KO) with or without expression of the GFP fused to the N-terminus of one GMC-oxidoreductase (Mr_2KO-GFP). Our results show the versatility of the protein content of the fibrils, with fibrils composed of different proteins inter- and even intra-clade, clades B and C viruses presenting fibrils with a protein composition closer to each other than that of clade A viruses.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-16_01:28:53.112.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041298
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Mimivirus; NCBI TaxID: 315393; scientific name: Acanthamoeba castellanii; NCBI TaxID: 5755;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-03 01:44:09	ID requested
⏵ 1	2024-06-16 01:28:53	announced

Alempic JM, Bisio H, Villalta A, Santini S, Lartigue A, Schmitt A, Bugnot C, Notaro A, Belmudes L, Adrait A, Poirot O, Ptchelkine D, De Castro C, Cout, é Y, Abergel C, Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes. Microlife, 5():uqae006(2024) [pubmed]

10.1093/femsml/uqae006;

10.6019/PXD041298;

submitter keyword: Mimivirus, fibrils, virion, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041298
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based proteomic analysis of virions and fibrils from Mimiviridae