PXD041298 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry-based proteomic analysis of virions and fibrils from Mimiviridae |
Description | Mimivirus 1.2Mb genome is organized into a 30 nm nucleocapsid-like structure made of two closely related GMC-oxidoreductases, also composing the fibrils decorating its virions. In this work, we used MS-proteomics to characterize the protein content of virions and fibrils from different members of the Mimiviridae family (clade A: Mimivirus reunion -Mr- and Mimivirus M4 -M4, clade B: Moumouvirus australiensis -Ma- and Moumouvirus maliensis -Mm, clade C: Megavirus chilensis -Mc- and Megavirus vitis -Mv). Furthermore, we analyzed fractions purified from Mr mutants devoid of one of the two GMC-oxidoreductases (Mr_KOqu_143 and Mr_KOqu_946), or of both GMC-oxidoreductases (Mr_2KO) with or without expression of the GFP fused to the N-terminus of one GMC-oxidoreductase (Mr_2KO-GFP). Our results show the versatility of the protein content of the fibrils, with fibrils composed of different proteins inter- and even intra-clade, clades B and C viruses presenting fibrils with a protein composition closer to each other than that of clade A viruses. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-16 |
AnnouncementXML | Submission_2024-06-16_01:28:53.112.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041298 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yohann Couté |
SpeciesList | scientific name: Mimivirus; NCBI TaxID: 315393; scientific name: Acanthamoeba castellanii; NCBI TaxID: 5755; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-03 01:44:09 | ID requested | |
⏵ 1 | 2024-06-16 01:28:53 | announced | |
Publication List
Alempic JM, Bisio H, Villalta A, Santini S, Lartigue A, Schmitt A, Bugnot C, Notaro A, Belmudes L, Adrait A, Poirot O, Ptchelkine D, De Castro C, Cout, é Y, Abergel C, Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes. Microlife, 5():uqae006(2024) [pubmed] |
10.1093/femsml/uqae006; |
10.6019/PXD041298; |
Keyword List
submitter keyword: Mimivirus, fibrils, virion, nanoLC-MS/MS |
Contact List
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
lab head | |
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041298
- Label: PRIDE project
- Name: Mass spectrometry-based proteomic analysis of virions and fibrils from Mimiviridae