PXD041297

PXD041297 is an original dataset announced via ProteomeXchange.

Title	C1improves motor performance and muscle pathology via activating AMPK-PGC-1α in a mouse model of Duchenne muscular dystrophy
Description	Objectives: This study was conducted to investigate the protective effect and intervention mechanism of C1 (a selective AMPK activator) on muscle damage in mice with Duchenne muscular dystrophy (DMD). Methods: We administered C1 (18 mg/kg and 54 mg/kg) to 10-week-old male DMD mice for 8 weeks. A series of behavioral tests were carried out to assess motor ability. Skeletal muscle samples were subjected to pathological analysis, western blot and proteomic analysis to explore the different protein networks associated with muscle pathology. Compound C, an AMPKα inhibitor, has been used as a negative control for follow-up experiments to verify the efficiency of C1 on AMPK activation. Key findings： C1 treatment significantly enhanced motor function and reduced muscle fibrosis and inflammation in DMD mice. We then found that C1 activated the AMPKα/PGC-1α signaling pathway, increasing ATP levels and mitochondrial DNA copy number in DMD mice. These improved effects on mitochondrial function were reversed by Compound C. In addition, Gastrocnemius muscle proteomics showed that C1 attenuated oxidative stress damage and improved muscle structural development. Conclusion: Collectively, C1 alleviates muscle damage in DMD mice by activating AMPKα to improve mitochondria.
HostingRepository	iProX
AnnounceDate	2023-04-03
AnnouncementXML	Submission_2023-04-03_00:33:21.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiao Yang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	trimethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-03 00:33:07	ID requested
⏵ 1	2023-04-03 00:33:21	announced

Dataset with its publication pending

submitter keyword: Duchenne muscular dystrophy, Fibrosis, Inflammation, Oxidative damage, Mitochondria, AMPK

Xifei Yang
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	xifeiyang@gmail.com
lab head
Xiao Yang
contact affiliation	University of South China
contact email	2584620446@qq.com
dataset submitter

iProX dataset URI