PXD041291

PXD041291 is an original dataset announced via ProteomeXchange.

Title	Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap
Description	In this work, a LPIT-reversed phase liquid chromatography-tandem mass spectrometry (LPIT-RPLC-MS/MS) platform was established for deep bottom-up proteomics. LPIT was used here as a robust and effective method for peptide fractionation. LPIT separates peptides based on their effective charges and hydrodynamic radii, which is orthogonal to that of RPLC. With excellent orthogonality, the integration of LPIT with RPLC-MS/MS could effectively increase the number of peptide and protein being detected. When analyzing HeLa cells, peptide and protein coverages were increased by ~89.2% and 50.3%, respectively. With high efficiency and low cost, this LPIT based peptide fraction method could potentially be used in routine deep bottom-up proteomics.
HostingRepository	iProX
AnnounceDate	2023-04-03
AnnouncementXML	Submission_2024-06-06_00:52:44.813.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinyan Fu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-02 21:58:31	ID requested
1	2023-04-02 21:58:45	announced
⏵ 2	2024-06-06 00:52:45	announced	2024-06-06: Update information.

Fu X, Hong J, Zhai Y, Liu K, Xu W, Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap. Anal Chem, 95(27):10137-10144(2023) [pubmed]

submitter keyword: bottom-up proteomics, liquid phase ion trap, mass spectrometry, peptide fractionation

Wei Xu
contact affiliation	Beijing Institute of Technology
contact email	weixu@bit.edu.cn
lab head
Xinyan Fu
contact affiliation	Beijing Institute of Technology
contact email	1121428828@qq.com
dataset submitter

iProX dataset URI