PXD041283

PXD041283 is an original dataset announced via ProteomeXchange.

Title	Comparison of the ability of exosomes and ectosomes derived from mesenchymal stem cells to promote cartilage regeneration in a rat osteochondral defect model
Description	Background: Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) offer promising prospects for stimulating cartilage regeneration. The different formation mechanisms suggest that exosomes and ectosomes possess different biological functions. However, little attention has been paid to the differential effects of EV subsets on cartilage regeneration. Methods: Our study compared the effects of the two EVs on chondrocytes and bone marrow–derived MSCs (BMSCs) in vitro and loaded them into type I collagen hydrogels to optimize EV application for the treatment of osteochondral defects in vivo. Results: In vitro experiments demonstrate that ASC-Exos significantly promoted the proliferation and migration of both cells more effectively than ASC-Ectos. Furthermore, ASC-Exos facilitated a stronger differentiation of BMSCs into chondrogenic cells than ASC-Ectos, but both inhibited chondrocyte apoptosis to a similar extent. In the osteochondral defect model of rats, ASC-Exos promoted cartilage regeneration in situ better than ASC-Ectos. At 8 weeks, the Gel+Exo group had higher macroscopic and histological scores, a higher value of BV/TV, a lower value of Tb.Sp, and a better remodeling of extracellular matrix than the Gel+Ecto group. At 4 and 8 weeks, the expression of CD206 and Arginase-1 in the Gel+Exo group was significantly higher than that in the Gel+Ecto group. Conclusion: Our findings indicate that administering ASC-Exos may be a more effective EV strategy for cartilage regeneration than the administration of ASC-Ectos.
HostingRepository	iProX
AnnounceDate	2023-04-01
AnnouncementXML	Submission_2024-02-22_18:22:15.046.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tengjing Xu
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-02 19:08:26	ID requested
1	2023-04-02 19:11:40	announced
⏵ 2	2024-02-22 18:22:15	announced	2024-02-23: Update information.

Xu T, Yu X, Xu K, Lin Y, Wang J, Pan Z, Fang J, Wang S, Zhou Z, Song H, Zhu S, Dai X, Comparison of the ability of exosomes and ectosomes derived from adipose-derived stromal cells to promote cartilage regeneration in a rat osteochondral defect model. Stem Cell Res Ther, 15(1):18(2024) [pubmed]

submitter keyword: Cartilage regeneration, Mesenchymal stem cells, Exosomes, Ectosomes, Macrophages.

Xuesong Dai
contact affiliation	The Second Affiliated Hospital, Zhejiang University School of Medicine
contact email	daixshz@zju.edu.cn
lab head
Tengjing Xu
contact affiliation	The Second Affiliated Hospital, Zhejiang University School of Medicine
contact email	12018466@zju.edu.cn
dataset submitter

iProX dataset URI