PXD041278

PXD041278 is an original dataset announced via ProteomeXchange.

Title	A One-Step Heating Process for Efficient Desalting and Purification of Proteomic Samples in Sample Pre-Treatment
Description	The conventional method for desalting columns in proteomic sample pre-treatment is a multi-step process and is susceptible to trace sample loss. This study presents a novel proteomic pre-treatment method that combines enzymatic digestion with ultrafiltration and desalting through a one-step heating process. The procedure involves washing protein samples with ammonium bicarbonate prior to enzymatic digestion, and subsequently removing ammonium bicarbonate from the resulting peptide solution by heating to yield pure, desalted peptides. This approach is simple to implement, does not result in sample loss or contamination from PEG polymers, and can be directly interfaced with LC-MS for subsequent mass spectrometry analysis.
HostingRepository	iProX
AnnounceDate	2023-03-30
AnnouncementXML	Submission_2024-06-11_19:04:57.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemeng Ji
SpeciesList	scientific name: Cronobacter sakazakii ATCC BAA-894; NCBI TaxID: 290339;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-02 02:49:37	ID requested
⏵ 1	2024-06-11 19:04:57	announced

Dataset with its publication pending

submitter keyword: Proteomic analysis, Sample pre-treatment, Desalting column, Heating, Ammonium bicarbonate

Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	jixuemeng@nankai.edu.cn
lab head
Xuemeng Ji
contact affiliation	Nankai Univeristy
contact email	018071@nankai.edu.cn
dataset submitter

iProX dataset URI