PXD041244

PXD041244 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Absolute quantification of peptides from TIM23 or PINK1-3HA using AQUA peptides
Description	To quantify TIM23 and PINK1-3HA in the immunoprecipitates, control or PINK1-3HA expressing HeLa cells were treated with CCCP and subjected to immunoprecipitation with anti-HA antibody bound to magnetic beads. After spiked with 200 fmol stable isotope-labeled AQUA peptides (Cosmo Bio Co Ltd) as standards, proteins on the beads were digested with 400 ng trypsin/LysC mixture at 37ºC overnight. The digests were reduced, alkylated, acidified, desalted, evaporated, and dissolved in 0.1% trifluoroacetic acid and 3% acetonitrile. The absolute amounts of tryptic peptides derived from TIM23 and PINK1-3HA were measured by PRM. Targeted MS/MS scans were acquired by a time-scheduled inclusion list at a resolution of 70,000, an AGC target of 2e5, an isolation window of 2.0 m/z, a maximum injection time of 300 ms, and a normalized collision energy of 27. Time alignment and quantification of the transitions of three biological replicates were performed using Skyline software.
HostingRepository	jPOST
AnnounceDate	2023-04-07
AnnouncementXML	Submission_2023-08-21_23:51:54.944.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; Acetyl; S-carboxamidomethyl-L-cysteine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-31 02:06:16	ID requested
1	2023-04-06 15:03:11	announced
⏵ 2	2023-08-21 23:51:55	announced	2023-08-22: Updated PubMed.

Publication List

Akabane S, Watanabe K, Kosako H, Yamashita SI, Nishino K, Kato M, Sekine S, Kanki T, Matsuda N, Endo T, Oka T, TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria. Cell Rep, 42(5):112454(2023) [pubmed]

Akabane S, Watanabe K, Kosako H, Yamashita SI, Nishino K, Kato M, Sekine S, Kanki T, Matsuda N, Endo T, Oka T, TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria. Cell Rep, 42(5):112454(2023) [pubmed]

Keyword List

submitter keyword: TIM23, PINK1, AQUA

submitter keyword: TIM23, PINK1, AQUA

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002110/