PXD041221

PXD041221 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana-Actinacidiphila interaction
Description	How bacteria from the microbiota modulate the physiology of its host is an important question to address. Previous work revealed that the metabolic status of Arabidopsis thaliana was crucial for the specific recruitment of Streptomycetaceae into the microbiota. Here, the Arabidopsis-Actinacidiphila interaction was further depicted by inoculating axenic Arabidopsis with Actinacidiphila cocklensis DSM 42063 or Actinacidiphila bryophytorum DSM 42138(previously named Streptomyces cocklensis and Streptomyces bryophytorum). We demonstrated that these two bacteria colonize A. thaliana wild-type plants, but their colonization efficiency was reduced in a chs5 mutant with defect in isoprenoid, phenylpropanoids and lipids synthesis. We observed that those bacteria affect the growth of the chs5 mutant but not of the wild-type plants. Using a mass spectrometry-based proteomic approach, we showed a modulation of the Arabidopsis proteome and in particular its components involved in photosynthesis or phytohormone homeostasis or perception by A. cocklensis and A. bryophytorum. This study unveils specific aspects of the Actinacidiphila-Arabidopsis interaction, which implies molecular processes impaired in the chs5 mutant and otherwise at play in the wild-type. More generally, this study highlights complex and distinct molecular interactions between Arabidopsis thaliana and bacteria belonging to the Actinacidiphila genus.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-29_06:52:13.207.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Magali Rompais
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 09:08:33	ID requested
⏵ 1	2024-01-29 06:52:13	announced

10.6019/PXD041221;

10.1094/PHYTOFR-12-22-0149-R;

submitter keyword: Arabidopsis thaliana, nanoAcquity (Waters), Q-Exactive Plus (Thermo), Actinacidiphila

Christine CARAPITO
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO) Département des Sciences Analytiques (DSA) Institut pluridisciplinaire Hubert Curien (IPHC) - UMR7178 Centre national de la recherche scientifique (CNRS) Université de Strasbourg (Unistra) France
contact email	ccarapito@unistra.fr
lab head
Magali Rompais
contact affiliation	IPHC-UMR7178-CNRS-UDS-LSMBO
contact email	magali.rompais@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD041221

PRIDE project URI

• PRIDE
  1. PXD041221
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana-Actinacidiphila interaction