PXD041209

PXD041209 is an original dataset announced via ProteomeXchange.

Title	The impact of culture conditions on E. coli acetylome and proteome
Description	A proteome and acetylome study under different culture conditions was carried out by quantitative label-free mass spectrometry analysis. E. coli K12 BW25113 strain was grown employing TB7 complex medium or M9 minimal medium, supplemented with glucose 20 mM or glycerol 40 mM as carbon source. Therefore, 4 culture conditions (TB7-glucose, TB7-glycerol, MM9-glucose, and MM9-glycerol) were used. Samples were taken in exponential and in stationary growth phase, resulting in 8 experimental samples: TB7-glucose in exponential phase, TB7-glucose in stationary phase, TB7-glycerol in exponential phase, TB7-glycerol in stationary phase, MM9-glucose in exponential phase, MM9-glucose in stationary phase, MM9-glycerol in exponential phase, and MM9-glycerol in stationary phase, with 4 biological replicates from each. This work emphasises the importance of the culture conditions choice, as they determine both, location of acetylation, and acetylation level, which may have an impact on regulation of the central metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-05-21
AnnouncementXML	Submission_2024-05-21_07:30:48.248.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Teresa de Diego Puente
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 04:22:06	ID requested
⏵ 1	2024-05-21 07:30:49	announced

10.1016/j.isci.2024.109017;

Lozano-Terol G, Chiozzi RZ, Gallego-Jara J, Sola-Mart, í, nez RA, Vivancos AM, Ortega Á, Heck AJR, D, í, az MC, de Diego Puente T, protein acetylome. iScience, 27(2):109017(2024) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: E. coli, LC-MS, PTM,Lysine acetylation, fluxes, metabolism regulation

Teresa de Diego Puente
contact affiliation	Departamento de Bioquímica y Biología Molecular B e Inmunologia. Facultad de Química. Universidad de Murcia. Campus de Excelencia Mare Nostrum
contact email	tdp@um.es
lab head
Teresa de Diego Puente
contact affiliation	Department of Biochemistry and Molecular Biology B and Immunology. Faculty of Chemistry. University of Murcia. EUniWell. Mare Nostrum Campus of Excellence.
contact email	tdp@um.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD041209

PRIDE project URI

• PRIDE
  1. PXD041209
     1. Label: PRIDE project
     2. Name: The impact of culture conditions on E. coli acetylome and proteome