PXD041203

PXD041203 is an original dataset announced via ProteomeXchange.

Title	Murine podocyte mitochondrial complexsome analysis
Description	Proximity labeling discovered a few mitochondrial cristae organizing proteins as NDUFS4 interacting protein. However proximity labeling is unable to differentiate between a direct interaction or a mere close association between NDUFS4 and cristae forming proteins. Additionally, it does not specify if the association of NDUFS4 with these proteins occurs in the context of individual complexes or RSCs. Consequently, to explore whether these cristae forming proteins comigrate and are retained within different complexes in RSCs, we performed complexsome profiling analysis on isolated mitochondria from HG-treated Ndufs4 overexpressed podocytes, with HG-treated cells as control. We separated ETC complexes with BN-PAGE, sliced five distinct bands representing distinct RSCs, labeled 1–5, after Coomassie blue staining, followed by mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2024-02-08
AnnouncementXML	Submission_2024-02-08_09:42:34.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antrix Jain
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 03:34:05	ID requested
⏵ 1	2024-02-08 09:42:35	announced

Dataset with its publication pending

submitter keyword: Complexsome, podocyte, mouse, respiratory supercomplex

Farhad R. Danesh
contact affiliation	Division of Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX
contact email	fdanesh@mdanderson.org
lab head
Antrix Jain
contact affiliation	Baylor College of Medicine
contact email	antrixj@bcm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041203
     1. Label: PRIDE project
     2. Name: Murine podocyte mitochondrial complexsome analysis