PXD041172

PXD041172 is an original dataset announced via ProteomeXchange.

Title	Immunoprecipitation analysis of Histomonas meleagridis
Description	The identification of the molecular mechanisms by which parasites and hosts interact with each other is crucial in understanding how parasites evade the host’s immune system and ultimately cause disease. Through immunoaffinity in association with high-throughput proteomic analysis, we defined the repertoire of antigenic targets involved in the antibody response to H. meleagridis. As such, we isolated immunogenic proteins of the protozoan by immunoprecipitation employing sera from chickens and turkeys, vaccinated and challenged, with an attenuated and virulent strain of H. meleagridis, respectively. Amongst all analyzed samples, a total of 111 proteins were identified as putative immunogenic proteins. Separating the data amongst the two hosts studied, 88 proteins were identified with chicken immune sera, and 67 with turkey sera. From the antigenic proteins identified with chicken immune sera, 54 were classified as proteins displaying putative antigenicity and 46 whilst using turkey immune sera.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:31:34.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivana BILIC
SpeciesList	scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-28 17:26:56	ID requested
⏵ 1	2025-05-06 09:31:35	announced

10.1038/s41598-025-88855-y;

de Jesus Ramires M, Hummel K, Hatfaludi T, Hess M, Bilic I, Host-specific targets of Histomonas meleagridis antigens revealed by immunoprecipitation. Sci Rep, 15(1):5800(2025) [pubmed]

submitter keyword: virulence, antigens, attenuation, immunoprecipitation, protozoa,immunoproteome, Histomonas meleagridis, comparative proteomic analysis, LC-MS/MS

Ivana Bilic
contact affiliation	University Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	ivana.bilic@vetmeduni.ac.at
lab head
Ivana BILIC
contact affiliation	Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna
contact email	ivana.bilic@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD041172

PRIDE project URI

• PRIDE
  1. PXD041172
     1. Label: PRIDE project
     2. Name: Immunoprecipitation analysis of Histomonas meleagridis