PXD041169

PXD041169 is an original dataset announced via ProteomeXchange.

Title	p97/VCP promotes the recycling of endocytic cargo
Description	The endocytic pathway is of central importance for eukaryotic cells, as it enables uptake of extracellular materials, membrane protein quality control and recycling, as well as modulation of receptor signaling. While the ATPase p97 (VCP, Cdc48) has been found to be involved in the fusion of early endosomes and endolysosomal degradation, its role in endocytic trafficking is still incompletely characterized. Here, we identify myoferlin (MYOF), a ferlin family member with functions in membrane trafficking and repair, as a hitherto unknown p97 interactor. The interaction of MYOF with p97 depends on the cofactor PLAA previously linked to endosomal sorting. Besides PLAA, shared interactors of p97 and MYOF comprise several proteins involved in endosomal recycling pathways, including Rab11, Rab14 and the transferrin receptor CD71. Accordingly, a fraction of p97 and PLAA localizes to MYOF-, Rab11-, and Rab14-positive endosomal compartments. Pharmacological inhibition of p97 delays transferrin recycling, indicating that p97 promotes not only the lysosomal degradation, but also the recycling of endocytic cargo.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:07.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Buchberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-28 10:26:38	ID requested
1	2023-09-28 10:22:25	announced
⏵ 2	2023-11-14 08:53:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: MYOF, myoferlin, VCP, Cdc48,p97, Rab11

Alexander Buchberger
contact affiliation	Chair of Biochemistry I, Biocenter, University of Wuerzburg
contact email	alexander.buchberger@uni-wuerzburg.de
lab head
Alexander Buchberger
contact affiliation	Chair of Biochemistry I, Biocenter, University of Wuerzburg
contact email	alexander.buchberger@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041169
     1. Label: PRIDE project
     2. Name: p97/VCP promotes the recycling of endocytic cargo