PXD041168

PXD041168 is an original dataset announced via ProteomeXchange.

Title	Acute deletion of the glucocorticoid receptor in hepatocytes disrupts postprandial lipid metabolism
Description	Hepatic lipid metabolism is highly dynamic, and disruption of several circadian transcriptional regulators results in hepatic steatosis. This includes genetic disruption of the glucocorticoid receptor (GR) as the liver develops. To address the functional role of GR in the adult liver, we used an acute hepatocyte-specific GR knockout model (hepGRKO) to study temporal hepatic lipid metabolism governed by GR at several pre- and postprandial timepoints. Lipidomics analysis revealed significant temporal lipid metabolism, with GR disruption resulting in impaired regulation of specific triglycerides, non-esterified fatty acids, and sphingolipids. This correlated with increased number and size of lipid droplets and mildly reduced mitochondrial respiration, most noticeably in the postprandial phase. Proteomics and transcriptomics analyses suggest that dysregulated lipid metabolism originates from pronounced induced expression of enzymes involved in fatty acid synthesis, -oxidation, and sphingolipid metabolism. Integration of GR cistromic data suggests that induced genes are a result of regulatory actions secondary to direct GR effects on transcription.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:02.505.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Boel Pedersen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-28 08:20:04	ID requested
1	2023-09-07 09:56:28	announced
⏵ 2	2023-11-14 09:05:05	announced	2023-11-14: Updated project metadata.

Correia CM, Pr, æ, stholm SM, Havelund JF, Pedersen FB, Siersb, æ, k MS, Ebbesen MF, Gerhart-Hines Z, Heeren J, Brewer J, Larsen S, Blagoev B, F, æ, rgeman NJ, Gr, ø, ntved L, Acute Deletion of the Glucocorticoid Receptor in Hepatocytes Disrupts Postprandial Lipid Metabolism in Male Mice. Endocrinology, 164(10):(2023) [pubmed]

submitter keyword: Glucocorticoid Receptor knockout mice, DIA,Mouse, Lipid Metabolism, Circadian rhythm, Liver, LC-MS/MS

Blagoy Blagoev
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact email	bab@bmb.sdu.dk
lab head
Felix Boel Pedersen
contact affiliation	University of Southern Denmark
contact email	felixbp@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD041168

PRIDE project URI

• PRIDE
  1. PXD041168
     1. Label: PRIDE project
     2. Name: Acute deletion of the glucocorticoid receptor in hepatocytes disrupts postprandial lipid metabolism