PXD041139

PXD041139 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals insufficient folliculogenesis in Aging Female associates with infertility
Description	The escalating issue of infertility has become a global concern. Female infertility is primarily attributed to the decrease in both the quantity and quality of oocytes with advancing age, which is a significant factor in female infertility. The present study aims to explore the proteomic profile of exosomes obtained from human follicular fluid to identify the protein signature associated with aging and unsuccessful in vitro fertilization (IVF) outcomes. Despite the lack of significant differences in the morphology and particle size of follicular fluid exosomes between the two groups, the proteomic analysis demonstrated a distinct pattern of differentially expressed proteins. B cell activation, pathogen invasion, and disrupted metabolic processes were found to be significantly higher in the aging group, indicating the involvement of these pathways in aging-associated infertility. Moreover, the PI3K-AKT signaling pathway, which is linked to follicle maturation, was identified as the most influential signaling pathway in the aging group. In vivo experiments corroborated the weakened role of exosomes derived from the aging group in follicle maturation. Finally, the study employed a random forest machine learning algorithm, which further suggested that hydrolase activity might play a crucial role in determining the final IVF outcome. The expression of key differentially expressed proteins was validated. This study provides novel insights into the aging-associated protein signature of follicular fluid exosomes and their potential role in infertility. The findings imply that the aging-related protein signature in exosomes could serve to improve the diagnosis and treatment of infertility.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:21:26.829.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-27 18:51:32	ID requested
⏵ 1	2025-05-06 09:21:27	announced

Liu Z, Zhou Q, Zan J, Tian J, Zhang Y, Wu F, Zhao H, Peng Q, Liu S, Chen Q, Liu E, Liao Z, Zou P, Mei L, Wang W, Dong S, Niu L, Wu S, He L, Zhou X, Jin Y, Li P, Yang S, Proteomic Analysis of Human Follicular Fluid-Derived Exosomes Reveals That Insufficient Folliculogenesis in Aging Women is Associated With Infertility. Mol Cell Proteomics, 24(4):100930(2025) [pubmed]

10.1016/j.mcpro.2025.100930;

submitter keyword: Aging,Proteomic, Follicle maturation, in vitro fertilization, Exosomes

Yang Sheng
contact affiliation	Reproductive Medicine Center, The Third Afliated Hospital of ShenZhen University
contact email	tobyys2000@aliyun.com
lab head
Zhen Liu
contact affiliation	Reproductive Medicine Center, The Third Afliated Hospital of ShenZhen University
contact email	liuzhen19910815@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041139
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals insufficient folliculogenesis in Aging Female associates with infertility