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PXD041139

PXD041139 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis reveals insufficient folliculogenesis in Aging Female associates with infertility
DescriptionThe escalating issue of infertility has become a global concern. Female infertility is primarily attributed to the decrease in both the quantity and quality of oocytes with advancing age, which is a significant factor in female infertility. The present study aims to explore the proteomic profile of exosomes obtained from human follicular fluid to identify the protein signature associated with aging and unsuccessful in vitro fertilization (IVF) outcomes. Despite the lack of significant differences in the morphology and particle size of follicular fluid exosomes between the two groups, the proteomic analysis demonstrated a distinct pattern of differentially expressed proteins. B cell activation, pathogen invasion, and disrupted metabolic processes were found to be significantly higher in the aging group, indicating the involvement of these pathways in aging-associated infertility. Moreover, the PI3K-AKT signaling pathway, which is linked to follicle maturation, was identified as the most influential signaling pathway in the aging group. In vivo experiments corroborated the weakened role of exosomes derived from the aging group in follicle maturation. Finally, the study employed a random forest machine learning algorithm, which further suggested that hydrolase activity might play a crucial role in determining the final IVF outcome. The expression of key differentially expressed proteins was validated. This study provides novel insights into the aging-associated protein signature of follicular fluid exosomes and their potential role in infertility. The findings imply that the aging-related protein signature in exosomes could serve to improve the diagnosis and treatment of infertility.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_09:21:26.829.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhen Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-27 18:51:32ID requested
12025-05-06 09:21:27announced
Publication List
Liu Z, Zhou Q, Zan J, Tian J, Zhang Y, Wu F, Zhao H, Peng Q, Liu S, Chen Q, Liu E, Liao Z, Zou P, Mei L, Wang W, Dong S, Niu L, Wu S, He L, Zhou X, Jin Y, Li P, Yang S, Proteomic Analysis of Human Follicular Fluid-Derived Exosomes Reveals That Insufficient Folliculogenesis in Aging Women is Associated With Infertility. Mol Cell Proteomics, 24(4):100930(2025) [pubmed]
10.1016/j.mcpro.2025.100930;
Keyword List
submitter keyword: Aging,Proteomic, Follicle maturation, in vitro fertilization, Exosomes
Contact List
Yang Sheng
contact affiliationReproductive Medicine Center, The Third Afliated Hospital of ShenZhen University
contact emailtobyys2000@aliyun.com
lab head
Zhen Liu
contact affiliationReproductive Medicine Center, The Third Afliated Hospital of ShenZhen University
contact emailliuzhen19910815@gmail.com
dataset submitter
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