PXD041121

PXD041121 is an original dataset announced via ProteomeXchange.

Title	ATM signaling delays skin pigmentation upon UV exposure by mediating MITF function towards DNA repair mode
Description	Skin pigmentation is paused following sun exposure, however the mechanism behind this pausing is unknown. Here we found that the UVB-induced DNA repair system, led by the ATM protein kinase, represses MITF transcriptional activity of pigmentation genes while placing MITF in DNA repair mode, thus directly inhibiting pigment production. Phosphoproteomics analysis revealed ATM to be the most significantly enriched pathway among all UVB-induced DNA repair systems. ATM inhibition in mouse or human skin, either genetically or chemically, induces pigmentation. Upon UVB, MITF transcriptional activation is blocked due to ATM dependent phosphorylation of MITF on S414, which modifies MITF activity and interactome towards DNA repair including binding to TRIM28 and RBBP4. Accordingly, MITF genome-occupancy is enriched in sites of high DNA damage that are likely repaired. This suggests that ATM harnesses the pigmentation key activator, for the necessary rapid, efficient DNA repair, thus optimizing the chances of the cell to survive.
HostingRepository	PRIDE
AnnounceDate	2023-07-19
AnnouncementXML	Submission_2023-07-19_06:37:56.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TamarZiv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-26 23:59:59	ID requested
⏵ 1	2023-07-19 06:37:56	announced

Dataset with its publication pending

submitter keyword: DNA repair,MITF

CarmitLevy
contact affiliation	Department of Human Genetics and Biochemistry Sackler School of Medicine Tel Aviv University Tel-Aviv, Israel
contact email	carmitlevy@post.tau.ac.il
lab head
TamarZiv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041121
     1. Label: PRIDE project
     2. Name: ATM signaling delays skin pigmentation upon UV exposure by mediating MITF function towards DNA repair mode