PXD041119

PXD041119 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAc crosstalks with ADP-ribosylation via PARG
Description	O-GlcNAc glycosylation is a prevalent protein post-translational modification (PTM) that occurs intracellularly. Previous investigations have demonstrated that O-GlcNAcylation crosstalks with phosphorylation and ubiquitination, but it is unclear whether it interplays with other PTMs. Here we studied its relationship with ADP-ribosylation, which involves decorating target proteins with the ADP-ribose moiety. We discovered that one ADP-ribosylation “eraser”- ADP-ribose glycohydrolase (PARG)- is O-GlcNAcylated at Ser26. O-GlcNAcylation of PARG is essential to maintain its nuclear localization and chromatin association. In hepatocellular carcinoma (HCC) cells, PARG O-GlcNAcylation enhances DNA damage-binding protein 1 (DDB1) poly(ADP-ribosyl)ation (PARylation) and attenuates its ubiquitination. DDB1 is thus stabilized and degrades its downstream targets, such as c-Myc. We further utilized mouse xenograft models and demonstrated that PARG-S26A promoted HCC. Our study thus revealed that PARG O-GlcNAcylation inhibits HCC, and we propose that O-GlcNAc glycosylation may crosstalk with many other PTMs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:28:01.967.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041119
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wen Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-26 23:10:41	ID requested
1	2024-01-29 09:30:44	announced
⏵ 2	2024-10-22 06:28:04	announced	2024-10-22: Updated project metadata.

10.1016/j.jbc.2023.105354;

Li J, Liu X, Peng B, Feng T, Zhou W, Meng L, Zhao S, Zheng X, Wu C, Wu S, Chen X, Xu X, Sun J, Li J, O-GlcNAc has crosstalk with ADP-ribosylation via PARG. J Biol Chem, 299(11):105354(2023) [pubmed]

10.6019/PXD041119;

submitter keyword: hepatocellular carcinoma, poly(ADP-ribosyl)ation,O-GlcNAcylation, PARG, c-Myc

Wen Zhou
contact affiliation	Peking University
contact email	wen.zhou@pku.edu.cn
lab head
Wen Zhou
contact affiliation	Peking University
contact email	wen.zhou@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD041119

PRIDE project URI

• PRIDE
  1. PXD041119
     1. Label: PRIDE project
     2. Name: O-GlcNAc crosstalks with ADP-ribosylation via PARG