PXD041098 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LiP-MS on HEK293 lysate treated with Probe Candidate TR05267290 and Negative Control TR05706192 |
Description | Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated HEK293 cell lysate with the candidate probe TR05267290 and the corresponding negative control TR05706192 to profile target engagement and other structural effects. This project is part of the IMI EUbOPEN project and was performed in collaboration with Kilian Huber (University of Oxford). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:02:22.645.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Viviane Reber |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-24 07:33:06 | ID requested | |
1 | 2023-09-01 10:10:24 | announced | |
⏵ 2 | 2024-10-22 06:02:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: target engagmenet profiling,LiP-MS |
Contact List
Dr. Matthias Gstaiger |
contact affiliation | Institute of Molecular Systems Biology, ETH Zurich |
contact email | gstaigem@ethz.ch |
lab head | |
Viviane Reber |
contact affiliation | IMSB, ETH Zurich |
contact email | reberv@ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD041098 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041098
- Label: PRIDE project
- Name: LiP-MS on HEK293 lysate treated with Probe Candidate TR05267290 and Negative Control TR05706192