PXD041091 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of PP2A-B55 targets in Drosophila uncovers regulation of Emerin during nuclear envelope reassembly |
Description | Entry into mitosis is triggered by mitotic kinases that phosphorylate multiple proteins to induce profound cellular changes including nuclear envelope breakdown, chromosome condensation and spindle assembly. Conversely, mitotic exit requires protein dephosphorylation as cells return to their interphase organization. Protein Phosphatase 2A in complex with its B55/Tws regulatory subunit (PP2A-B55) is known to play a crucial role in this transition. However, the precise events and substrates that it regulates are incompletely understood. We used proteomic approaches in Drosophila to identify proteins that interact with PP2A-B55 and are dephosphorylated in a PP2A-B55 dependent manner. Among several candidates, we identified Otefin (also known as Emerin) as a target of PP2A-B55 in the process of nuclear envelope reformation after mitosis. Emerin is a protein of the inner nuclear membrane that interacts with the DNA-binding protein Barrier-to-Autointegration Factor (BAF) via a LEM domain. Our results indicate that phosphorylation of Emerin at Ser50 and Ser54 near its LEM domain negatively regulates its association with BAF, Lamin and additional Emerin. Using live imaging, we show that dephosphorylation of Emerin at the identified sites determines the timing of nuclear envelope reformation. Genetic rescue experiments indicate that this regulation is required in vivo during embryonic development. Phosphoregulation of the Emerin-BAF complex formation by PP2A-B55 appears as a key event of mitotic exit that is likely to be conserved across species. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:04:39.929.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041091 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Eric Bonneil |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-24 04:35:59 | ID requested | |
1 | 2023-08-10 06:54:32 | announced | |
⏵ 2 | 2023-11-14 09:04:40 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD041091; |
Emond-Fraser V, Larouche M, Kubiniok P, Bonneil É, Li J, Bourouh M, Frizzi L, Thibault P, Archambault V, . Open Biol, 13(7):230104(2023) [pubmed] |
Keyword List
submitter keyword: Otefin, PP2A, BAF, Lamin,Nuclear Envelope, Mitotic exit, Mitosis, Emerin |
Contact List
Vincent Archambault |
contact affiliation | IRIC-Université de Montréal |
contact email | vincent.archambault@umontreal.ca |
lab head | |
Eric Bonneil |
contact affiliation | Proteomic Platform |
contact email | eric.bonneil@umontreal.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041091
- Label: PRIDE project
- Name: Identification of PP2A-B55 targets in Drosophila uncovers regulation of Emerin during nuclear envelope reassembly