PXD041089

PXD041089 is an original dataset announced via ProteomeXchange.

Title	LiP-MS on Purified CAMKK1 treated with Staurosporine
Description	Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating protein with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated protein sample. The protease preferentially cleaves accessible and flexible regions of the protein, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated affinity-purified CAMKK1 kinase inhibitor Staurosporine. The data were generated as part of the IMI EUbOPEN project.
HostingRepository	PRIDE
AnnounceDate	2023-04-26
AnnouncementXML	Submission_2023-04-26_06:29:12.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VivianeReber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-24 04:07:01	ID requested
⏵ 1	2023-04-26 06:29:12	announced

Dataset with its publication pending

submitter keyword: target engagement,LiP-MS

Dr. MatthiasGstaiger
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich
contact email	gstaigem@ethz.ch
lab head
VivianeReber
contact affiliation	IMSB, ETH Zurich
contact email	reberv@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041089
     1. Label: PRIDE project
     2. Name: LiP-MS on Purified CAMKK1 treated with Staurosporine