PXD041087

PXD041087 is an original dataset announced via ProteomeXchange.

Title	miR-1307-5p counteracts breast cancer angiogenesis and metastasis
Description	MiRNAs have been shown to alter both protein expression and secretion in different cellular contexts. By combining in vitro, in vivo and in silico techniques, we demonstrated that overexpression of pre-miR-1307 reduced the ability of breast cancer cells to induce endothelial cell sprouting and angiogenesis. However, the molecular mechanism behind this and the effect of the individual mature miRNAs derived from pre-miR-1307 on protein secretion and is largely unknown. Here, we overexpressed miR-1307-3p|0, -3p|1 and 5p|0 in MDA-MB-231 breast cancer cells and assessed the impact of miRNA overexpression on protein secretion by Mass Spectrometry. Unsupervised hierarchical clustering revealed a distinct phenotype induced by overexpression of miR-1307-5p|0 compared to the controls and to the 5’isomiRs derived from the 3p-arm. Together, our results suggest different impacts of miR-1307-3p and miR-1307-5p on protein secretion which is in line with our in vitro observation that miR-1307-5p, but not the isomiRs derived from the 3p-arm reduce endothelial cell sprouting in vitro. Hence these data support the hypothesis that miR-1307-5p is at least partly responsible for impaired vasculature in tumors overexpressing pre-miR-1307.
HostingRepository	PRIDE
AnnounceDate	2024-12-11
AnnouncementXML	Submission_2024-12-11_07:24:35.622.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Schwarzmüller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-24 03:12:39	ID requested
⏵ 1	2024-12-11 07:24:35	announced

Dataset with its publication pending

submitter keyword: breast cancer, cell culture supernatant, DIA, MDA-MB-231, SP3, miR-1307,human, secretome

Stefan Wiemann
contact affiliation	Prof. Dr. Stefan Wiemann Molekulare Genomanalyse (B050) Deutsches Krebsforschungszentrum Im Neuenheimer Feld 580 69120 Heidelberg Tel: +49 6221 42 4646 Fax: +49 6221 42 3454
contact email	s.wiemann@dkfz-heidelberg.de
lab head
Luisa Schwarzmüller
contact affiliation	Division of Molecular Genome Analysis German Cancer Research Center (DKFZ)
contact email	luisa.schwarzmueller@gmx.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD041087

PRIDE project URI

• PRIDE
  1. PXD041087
     1. Label: PRIDE project
     2. Name: miR-1307-5p counteracts breast cancer angiogenesis and metastasis