PXD041070

PXD041070 is an original dataset announced via ProteomeXchange.

Title	A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3
Description	Type I interferons (IFNs) are consequential cytokines in antibacterial defense. Whether and how bacterial pathogens inhibit innate immune receptor-driven type I IFN expression remains mostly unknown. By screening a library of enterohemorrhagic Escherichia coli (EHEC) mutants, we uncovered EhaF, an uncharacterized protein, as an inhibitor of innate immune responses including IFNs. Further analyses identified EhaF as a secreted autotransporter—a type of bacterial secretion system with no known innate immune-modulatory function—that translocates into host cell cytosol and inhibit IFN response to EHEC. Mechanistically, EhaF interacts with and inhibits the MiT/TFE family transcription factor TFE3 resulting in impaired TANK phosphorylation and consequently, reduced IRF3 activation and type I IFN expression. Notably, EhaF-mediated innate immune suppression promotes EHEC colonization and pathogenesis in vivo. Overall, this study has uncovered a previously unknown autotransporter-based bacterial strategy that targets a specific transcription factor to subvert innate host defense.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:55.787.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeremy Balsbaugh
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-23 12:04:10	ID requested
1	2023-04-13 04:16:22	announced
⏵ 2	2023-11-14 08:44:56	announced	2023-11-14: Updated project metadata.

Ta A, Ricci-Azevedo R, Vasudevan SO, Wright SS, Kumari P, Havira MS, Surendran Nair M, Rathinam VA, Vanaja SK, A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3. Nat Commun, 14(1):2035(2023) [pubmed]

submitter keyword: autotransporter, transcription factor, immune response, TFE3, escherichia coli

Jeremy Balsbaugh
contact affiliation	Proteomics & Metabolomics Facility, University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliation	University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041070
     1. Label: PRIDE project
     2. Name: A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3