PXD041041

PXD041041 is an original dataset announced via ProteomeXchange.

Title	Serum O-glycoproteome using O-glycopeptide truncation strategy
Description	Here, we report an O-glycopeptide truncation strategy for the characterization of protein O-GalNAcylation in biological samples. The O-glycopeptide truncation strategy utilizes another protease or O-glycopeptidase for targeted cleavage of tryptic and enriched O-glycopeptides, which increases the analytical coverage of O-GalNAc glycopeptides and glycoproteins. Tryptic O-glycopeptides covered with dense O-glycan clusters and terminal sialic acids could be well isolated by the hydrophilic-based enrichment approaches. And the enriched O-glycopeptides are then enzymatically truncated into shorter or less multiply O-glycosylated peptides, which are more favorable for MS detection and database search in general bottom-up glycoproteomics. We exploit different enzymatic schemes in the O-glycopeptide truncation strategy for large-scale O-glycoproteome analysis. Ultimately, we comprehensively identify nearly 2000 O-glycopeptides from 391 glycoproteins in a total of 75 µL human serum. Moreover, the truncated O-glycopeptides contribute to site-specific O-glycosylation characterization in stepped high-energy collisional dissociation fragmented LC-MS/MS. Together, the O-glycopeptide truncation strategy has great potential to facilitate the in-depth study of O-GalNAc glycoproteomics in biological samples.
HostingRepository	jPOST
AnnounceDate	2023-05-13
AnnouncementXML	Submission_2023-05-12_22:49:28.882.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luyao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-23 02:57:45	ID requested
⏵ 1	2023-05-12 22:49:29	announced

Dataset with its publication pending

submitter keyword: human serum, O-GalNAc glycosylation

Luyao Liu, Mingliang Ye
lab head
Luyao
contact affiliation	CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS)
dataset submitter

jPOST dataset URI

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