PXD041028 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HIV-1 Nef changes the proteome of T cells extracellular vesicles |
Description | Extracellular vesicles (EVs) are biomolecule carriers for intercellular communication in health and disease. Nef is a human immunodeficiency virus (HIV) virulence factor that is released from cells within EVs and is present in plasma EVs of HIV-1 infected individuals. We performed a quantitative proteomic analysis to fully characterize the Nef-induced changes in protein composition of T cell-derived EVs and identify novel host targets of HIV. Several proteins with well-described roles in infection or not previously associated with HIV pathogenesis were specifically modulated by Nef in EVs. Among the downregulated proteins are the interferon-induced transmembrane 1, 2 and 3 proteins (IFITM1-3), broad-spectrum antiviral factors known to be cell-to-cell transferable by EVs. Our data establish Nef as a modulator of EVs' global protein content and as an HIV factor that antagonizes IFITMs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:28:05.213.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adriana Franco Paes Leme |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-22 06:01:58 | ID requested | |
1 | 2024-01-30 02:42:30 | announced | |
⏵ 2 | 2024-10-22 06:28:06 | announced | 2024-10-22: Updated project metadata. |
Publication List
da Silva-Janu, á, rio ME, da Costa CS, Tavares LA, Oliveira AK, Janu, á, rio YC, de Carvalho AN, Cassiano MHA, Rodrigues RL, Miller ME, Palameta S, Arns CW, Arruda E, Paes Leme AF, daSilva LLP, HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors. Mol Cell Proteomics, 22(12):100676(2023) [pubmed] |
10.1016/j.mcpro.2023.100676; |
Keyword List
submitter keyword: HIV-1, Nef, exosomes, Extracelular vesicles |
Contact List
Luis L P daSilva |
contact affiliation | Centro de Pesquisa em Virologia (CPV) and Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil |
contact email | lldasilva@fmrp.usp.br |
lab head | |
Adriana Franco Paes Leme |
contact affiliation | CNPEM |
contact email | adriana.paesleme@lnbio.cnpem.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041028
- Label: PRIDE project
- Name: HIV-1 Nef changes the proteome of T cells extracellular vesicles