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PXD041028

PXD041028 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHIV-1 Nef changes the proteome of T cells extracellular vesicles
DescriptionExtracellular vesicles (EVs) are biomolecule carriers for intercellular communication in health and disease. Nef is a human immunodeficiency virus (HIV) virulence factor that is released from cells within EVs and is present in plasma EVs of HIV-1 infected individuals. We performed a quantitative proteomic analysis to fully characterize the Nef-induced changes in protein composition of T cell-derived EVs and identify novel host targets of HIV. Several proteins with well-described roles in infection or not previously associated with HIV pathogenesis were specifically modulated by Nef in EVs. Among the downregulated proteins are the interferon-induced transmembrane 1, 2 and 3 proteins (IFITM1-3), broad-spectrum antiviral factors known to be cell-to-cell transferable by EVs. Our data establish Nef as a modulator of EVs' global protein content and as an HIV factor that antagonizes IFITMs.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:28:05.213.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdriana Franco Paes Leme
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-22 06:01:58ID requested
12024-01-30 02:42:30announced
22024-10-22 06:28:06announced2024-10-22: Updated project metadata.
Publication List
da Silva-Janu, á, rio ME, da Costa CS, Tavares LA, Oliveira AK, Janu, á, rio YC, de Carvalho AN, Cassiano MHA, Rodrigues RL, Miller ME, Palameta S, Arns CW, Arruda E, Paes Leme AF, daSilva LLP, HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors. Mol Cell Proteomics, 22(12):100676(2023) [pubmed]
10.1016/j.mcpro.2023.100676;
Keyword List
submitter keyword: HIV-1, Nef, exosomes, Extracelular vesicles
Contact List
Luis L P daSilva
contact affiliationCentro de Pesquisa em Virologia (CPV) and Departamento de Biologia Celular e Molecular, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
contact emaillldasilva@fmrp.usp.br
lab head
Adriana Franco Paes Leme
contact affiliationCNPEM
contact emailadriana.paesleme@lnbio.cnpem.br
dataset submitter
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Dataset FTP location
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PRIDE project URI
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