PXD041018

PXD041018 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Investigating the role of METTL8 in sustaining GBM hallmarks
Description	Methyltransferase-like 8 (METTL8) is a RNA methyltransferase that plays a crucial role in mitochondrial translation. Previous study reported that METTL8 is overexpressed in cancer, but whether it contributes to GBM pathogenesis has not been explored. Here, we find that METTL8 is overexpressed in GBM stem cells (GSC) relative to non-cancerous human neural progenitor cells and astrocytes. METTL8 depletion significantly reduces GSC tumorsphere formation, differentiation potential, invasiveness and tumorigenicity. As expected, METTL8 is localized to the mitochondrial matrix of GSC. Although it is established that METTL8 binds to tRNAs, the protein interactors of METTL8 remains uncharacterized. To this end, we aim to define the METTL8 interactome using mitochondrial fractions of 293T cells, and then validate the identified interactors in GSC.
HostingRepository	jPOST
AnnounceDate	2024-03-22
AnnouncementXML	Submission_2024-03-21_08:00:13.450.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; L-methionine sulfoxide; deamidated L-glutamine; deamidated L-asparagine
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-21 23:05:31	ID requested
⏵ 1	2024-03-21 08:00:13	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: METTL8, glioblastoma, RNA methyltransferase, mitochondrion

submitter keyword: METTL8, glioblastoma, RNA methyltransferase, mitochondrion

Contact List

Derrick Ong Sek Tong
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002087/