PXD040998

PXD040998 is an original dataset announced via ProteomeXchange.

Title	EPIGENETIC MODULATION IN LIVE CELLS USING A PEPTIDE OF THE C-TERMINAL UNSTRUCTURED TAIL OF H2A.Z
Description	H2A.Z-nucleosomes participate in both euchromatic and heterochromatic scenarios and it has proven difficult to reveal correlation of the disparate roles with the stability features imparted by H2A.Z on the nucleosomes. Using a robust in situ assay of nucleosome stability and cell lines expressing only engineered forms of the variant we show that a major fraction of H2A.Z is released from the nucleosomes of peripheral heterochromatin at unusually high salt concentration, an observation reproduced with reconstituted nucleosomes. In cells expressing H2A.Z lacking its C-terminal tail, or upon addition of the tail peptide to control nuclei, canonical stability features are restored, the peripheral heterochromatin becomes dispersed and an increase of nuclease sensitivity, mainly at lamina-associated domains, ensues. Binding of the peptide to nucleosomes was detected by fluorescence correlation spectrometry. The peptide, when introduced into live cells, also induces reorganization of chromatin and decreases MYC expression, offering means for targeted epigenetic modulation.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:19:52.184.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uladzislau Vadadokhau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-21 01:41:29	ID requested
⏵ 1	2025-05-06 09:19:53	announced

10.1038/s41467-024-53514-9;

Imre L, N, á, n, á, si P, Benhamza I, Enyedi KN, Mocs, á, r G, Bosire R, Heged, ü, s É, Niaki EF, Cs, ó, ti Á, Darula Z, Cs, ő, sz É, P, ó, liska S, Scholtz B, Mez, ő G, Bacs, ó Z, Timmers HTM, Kusakabe M, Bal, á, zs M, V, á, mosi G, Ausio J, Cheung P, T, ó, th K, Tremethick D, Harata M, Szab, ó G, Epigenetic modulation via the C-terminal tail of H2A.Z. Nat Commun, 15(1):9171(2024) [pubmed]

submitter keyword: histone, nucleosome stability,epigenetics

Csősz Éva
contact affiliation	Proteomics Core Facility, Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary
contact email	cseva@med.unideb.hu
lab head
Uladzislau Vadadokhau
contact affiliation	University of Debrecen
contact email	vadadokhau@mailbox.unideb.hu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD040998

PRIDE project URI

• PRIDE
  1. PXD040998
     1. Label: PRIDE project
     2. Name: EPIGENETIC MODULATION IN LIVE CELLS USING A PEPTIDE OF THE C-TERMINAL UNSTRUCTURED TAIL OF H2A.Z