PXD040987

PXD040987 is an original dataset announced via ProteomeXchange.

Title	AI-facilitated multi-omics reveal TRF-controlled propionylation in determining protein homeostasis
Description	Firstly, we systematically identified rhythmically modified Kpr sites, through time-series propionylomic profiling of fly heads. Wild-type (WT) and per0 flies were entrained under light/dark cycles (LD) and then released into constant darkness (DD), respectively. On the first day of DD, whole heads of WT flies were collected every 6 hours, including Zeitgeber time 0 (ZT0), ZT6, ZT12 and ZT18, whereas per0 fly heads were collected at ZT6 and ZT18, respectively. For each sample, whole-cell lysates were digested by trypsin and labeled with one of the tandem mass tag (TMT) 6-plex reagents. Then, propionylated peptides were isolated by immuno-affinity enrichment using the pan-propionylation antibody. The 6 samples were mixed with equal amounts and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Secondly, metabolomic profiling was conducted using TRF-WT and TRF-H2BK17A fly heads collected at ZT9 and ZT21. After the preprocessing including homogenization and different reagents mixture followed by centrifugation. All samples were analyzed by the LC-MS/MS system. Additionally, Immunoprecipitaion and LC-MS/MS analysis was perform with fly heads using H2BK17pr antibody to evaluate the its enrichment capacity.
HostingRepository	iProX
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-05-22_00:45:08.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenwei Wang
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus; X500R QTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-20 19:20:13	ID requested
1	2023-03-20 19:20:51	announced
⏵ 2	2023-05-22 00:45:09	announced	2023-05-22: Update publication information.

Shui K, Wang C, Zhang X, Ma S, Li Q, Ning W, Zhang W, Chen M, Peng D, Hu H, Fang Z, Guo A, Gao G, Ye M, Zhang L, Xue Y, Small-sample learning reveals propionylation in determining global protein homeostasis. Nat Commun, 14(1):2813(2023) [pubmed]

submitter keyword: Drosophila, propionylation, metabolome, per

Yu Xue
contact affiliation	Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology and the Collaborative Innovation Center for Brain Science, Huazhong University of Science and Technology
contact email	xueyu@hust.edu.cn
lab head
Chenwei Wang
contact affiliation	Huazhong University of Science and Technology
contact email	reviewerfly2018@gmail.com
dataset submitter

iProX dataset URI