PXD040962

PXD040962 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Immune Isoform Atlas: Landscape of alternative splicing in human immune cells
Description	This dataset contains the global and terminal proteome of THP-1 and LCL cell lines untreated or treated with phorbol 12-myristate 13-acetate (PMA) and interferon-α2 (IFN-α2), respectively. Extracted proteins were digested with trypsin or V8 protease and protein terminal peptides were isolated with strong cation exchange chromatography or metal oxide-based ligand-exchange chromatography. All peptides were labeled with TMT-tag, and a total of 16 channels were used with quadruplicates of untreated LCL, IFN-α2-treated LCL, untreated THP-1, and PMA-treated THP-1 peptides, respectively. The labeled peptides were fractionated into 24 vials by reversed-phase HPLC at high pH conditions.
HostingRepository	jPOST
AnnounceDate	2024-04-08
AnnouncementXML	Submission_2024-04-08_10:12:25.607.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroshi Nishida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification; Acetyl; unknown modification; unknown modification
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-19 00:27:39	ID requested
⏵ 1	2024-04-08 10:12:25	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: alternative splicing, long-read sequencing, immune cells, transposable elements, translational efficiency, quantitative trait loci

submitter keyword: alternative splicing, long-read sequencing, immune cells, transposable elements, translational efficiency, quantitative trait loci

Contact List

Yasushi Ishihama
lab head
Hiroshi Nishida
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001823/