PXD040944 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | VRK1 regulates sensitivity to oxidative stress by altering the nuclear phosphoproteome and histone epigenetic modifications |
Description | Chromatin organization and its dynamic remodeling determine its accessibility and sensitivity to DNA damage. The major source of endogenous DNA damage is oxidative stress. We studied the role of the VRK1 chromatin kinase in the response to oxidative stress, which alters the nuclear phosphoproteome and the histone epigenetic pattern. Oxidative stress causes an accumulation of 8-oxoG DNA lesions that were significantly increased by VRK1 depletion, causing a significant accumulation of DNA strand breaks detected by their labeling with TUNEL assays. The analysis of the nuclear phosphoproteome in response to oxidative stress detected an enrichment of phosphorylated proteins associated with chromosome organization and chromatin remodeling, and these protein phosphorylations significantly decreased after VRK1 depletion. Because chromatin remodeling requires covalent modifications in the histone tails, we studied the effect of oxidative stress on the pattern of histones H4 and H3 epigenetic modifications. Oxidative stress induced the acetylation of H4 in K16, an increase of H3K9 acetylation, and a loss of H3K4me3. Depletion of VRK1 altered all these modifications induced by oxidative stress and resulted in the loss of H4K16ac and H3K9ac and an increase in H3K9me3 and H3K4me3 levels. All these changes induced by oxidative stress in the epigenetic pattern of histones are impaired by the depletion of VRK1, indicating that VRK1 plays a major role in the functional reorganization of chromatin in the response to oxidative stress. VRK1 depletion alters the nuclear phosphoproteome and the histone epigenetic pattern in response to oxidative stress. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:42:43.027.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thang Pham |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-17 07:48:02 | ID requested | |
1 | 2024-05-24 02:50:33 | announced | |
⏵ 2 | 2024-10-22 06:42:43 | announced | 2024-10-22: Updated project metadata. |
Publication List
Navarro-Carrasco E, Monte-Serrano E, Campos-D, í, az A, Rolfs F, de Goeij-de Haas R, Pham TV, Piersma SR, Gonz, á, lez-Alonso P, Jim, é, nez CR, Lazo PA, VRK1 Regulates Sensitivity to Oxidative Stress by Altering Histone Epigenetic Modifications and the Nuclear Phosphoproteome in Tumor Cells. Int J Mol Sci, 25(9):(2024) [pubmed] |
10.3390/ijms25094874; |
Keyword List
submitter keyword: methylation, acetylation, nuclear proteins,VRK1, oxidative stress, phosphoproteomics, chromatin |
Contact List
Connie Jimenez |
contact affiliation | OncoProteomics Laboratory, Amsterdam UMC, The Netherlands |
contact email | c.jimenez@amsterdamumc.nl |
lab head | |
Thang Pham |
contact affiliation | Amsterdam UMC |
contact email | t.pham@amsterdamumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040944
- Label: PRIDE project
- Name: VRK1 regulates sensitivity to oxidative stress by altering the nuclear phosphoproteome and histone epigenetic modifications